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MABN2724-100UG Anti-Sonic Hedgehog/Shh Antibody, clone 5E1

Anti-Sonic Hedgehog/Shh, clone 5E1, Cat. No. MABN2724, a mouse monoclonal antibody that detects Sonic Hedgehog and is tested in Western Blotting, Electron Microscopy, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, and Inhibition assay.

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100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABN2724-100UG
DescriptionAnti-Sonic Hedgehog/Shh Antibody, clone 5E1
Alternate Names
  • SHH
  • Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains
  • ShhNC
  • EC:3.1.
Background InformationSonic hedgehog protein (UniProt: Q63673; also known as SHH, EC:3.1.-.-, ShhNC, ShhN) is encoded by the Shh (also known as Vhh-1 ) gene (Gene ID: 29499) in rat. The Sonic Hedgehog (Shh) protein is a member of the hedgehog family of signaling molecules which also include Indian Hh and Desert Hh. It is initially synthesized as a precursor protein. The C-terminal domain of the precursor SHH displays an autoproteolysis activity and a cholesterol transferase activity, causing a cleavage of the precursor SHH into an N-terminal fragment (ShhN-product; aa 25-198) as well as the covalent attachment of a cholesterol moiety to the C-terminal end of the N-product. The N-product is the active signaling species, whereas the C-product has no signaling activity. Shh expression is highly regulated and varies depending on the developmental stage and tissue type. In the developing embryo, Shh is expressed in the notochord, floor plate of the neural tube, limb buds, and various other tissues. In adults, Shh expression is more restricted but remains important for tissue homeostasis and repair. The precursor protein is processed in the endoplasmic reticulum and Golgi apparatus before being transported to the cell membrane. The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers. Dysregulation of Shh signaling can lead to various congenital disorders and diseases. The intercellular signaling triggered by SHH is essential for a variety of patterning events during development including the development of the notochord, neural tube and plate, as well as the polarizing of the anterior-posterior axis of developing limb buds. Because of its importance in patterning during development, defects in SHH are the cause of a number of abnormalities including structural eye and brain abnormalities, teeth and bone malformations and finally limb and bone developmental defects. (Ref.: Reeber, S.L., et al. (2008). J Neurosci. 28(35):8698-708; Maun, H.R., et al. (2010). 285(34):26570-80; Voronova, A., et al. (2013). J Biol Chem. 288(6):4389-404; Coffman, L.G., et al. (2016). Oncotarget. 7(6):6916-32; Eitan, E., et al. (2016). Biol Open. 5(8):1086-92; Lewis, W.R., et al. (2016). PLoS Genet. 12(7):e1006220; Orquera, D.P., et al. (2016). Dev Biol. 416(1):212-224; Lusche, D.F., et al. (2019). MAbs. 11(4):691-708.)
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-Sonic Hedgehog/Shh, clone 5E1, Cat. No. MABN2724, a mouse monoclonal antibody that detects Sonic Hedgehog and is tested in Western Blotting, Electron Microscopy, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, and Inhibition assay.
Key Applications
  • Western Blotting
  • Electron Microscopy
  • Immunohistochemistry
  • Immunofluorescence
  • Immunocytochemistry
  • Inhibition
Application NotesTested Applications

Inhibition Assay: A representative lot inhibited Sonic hedgehog signaling (Maun, H.R., et al. (2010). 285(34):26570-80; Voronova, A., et al. (2013). J Biol Chem. 288(6):4389-404; Lusche, D.F., et al. (2019). MAbs. 11(4):691-708).

Immunocytochemistry Analysis: A representative lot detected Sonic hedgehog in Immunocytochemistry applications (Eitan, E., et al. (2016). Biol Open. 5(8):1086-92).

Immunofluorescence Analysis: A representative lot detected Sonic hedgehog in Immunofluorescence applications (Lewis, W.R., et al. (2016). PLoS Genet. 12(7):e1006220; Orquera, D.P., et al. (2016). Dev Biol. 416(1):212-224).

Immunohistochemistry Applications: A representative lot detected Sonic Hedgehog/Shh in Immunohistochemistry applications (Reeber, S.L., et al. (2008). J Neurosci. 28(35):8698-708; Maun, H.R., et al. (2010). 285(34):26570-80; Orquera, D.P., et al. (2016). Dev Biol. 416(1):212-224).

Western Blotting Analysis: A representative lot detected Sonic Hedgehog/Shh in Western Blotting applications (Coffman, L.G., et al. (2016). Oncotarget. 7(6):6916-32; Eitan, E., et al. (2016). Biol Open. 5(8):1086-92).

Electron Microscopy: A representative lot detected Sonic Hedgehog/Shh in Electron Microscopy applications (Eitan, E., et al. (2016). Biol Open. 5(8):1086-92).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenN-terminal, recombinant fragment of rat Sonic Hedgehog protein.
EpitopeN-terminal half
Clone5E1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5E1 is a mouse monoclonal antibody that specifically detects Sonic Hedgehog protein. It targets an epitope within 22 amino acids from the N-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Chicken
  • Rat
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat, Chicken.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Shh
Purification MethodProtein G purified
UniProt Number
Molecular WeightTarget molecular weight ~47kDa observed; 47.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat entorhinal cortex (EC) tissue lysate.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Sonic Hedgehog in rat entorhinal cortex (EC) tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2724-100UG 04065272163675