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ABS461 Anti-SHOC2 Antibody

Detect Leucine-rich repeat protein SHOC-2 using this rabbit polyclonal antibody, Anti-SHOC2 Antibody validated for use in western blotting & IHC.

Anti-SHOC2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: SHOC2 UniProt Number: Q9UQ13
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ABS461
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS461
DescriptionAnti-SHOC2 Antibody
Alternate Names
  • Leucine-rich repeat protein SHOC-2
  • Ras-binding protein Sur-8
  • SOC2
  • Protein soc-2 homolog
  • Protein sur-8 homolog
Background InformationThe Leucine-rich repeat protein SHOC-2 (SHOC2) is the regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and plays a key role in MAPK pathway activation. Upon RAS activation, SHOC2 acts as a positive modulator of the RAS-MAPK signaling via specifically allowing PP1c to dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity. SHOC2 localizes to cytoplasm when inactive but rapidly translocates to the nucleus when activated, such as during growth factor stimulation. Thus, as expected, SHOC2 also participates in FGF receptor signaling. Molecularly, SHOC2 is a scaffold protein that brings together activated Ras (activated via G-protein coupled receptors and the interaction of GTP to GDP) and Raf, which then facilitates the transfer of the phospho-Raf phosphate to other proteins for the activation of the MAP Kinase pathway for gene activation. Defects in SHOC2 lead to a type of multi-symptomatic disorder termed the Noonan Syndrome Loss of Anagen Hair (NSLAH) that affects everything from skeletal formation to facial features and many organs including the brain, skin, muscles, and heart. EMD Millipore’s Anti-SHOC2 rabbit polyclonal antibody has been tested in western blotting and immunohistochemistry in human samples.
References
Product Information
FormatAffinity Purified
Control
  • Jurkat cell lysate
PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Leucine-rich repeat protein SHOC-2 using this rabbit polyclonal antibody, Anti-SHOC2 Antibody validated for use in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: 5 µg/mL from a representative lot detected SHOC2 in human spleen tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human SHOC2.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Gene Symbol
  • SHOC2
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~65 kDa observed. Uniprot describes 2 isoforms at ~60 kDa and ~65 kDa. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SHOC2 in 15 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS461 04053252905728