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ABS1494-25UG Anti-SCYL1

Anti-SCYL1, Cat. No. ABS1494, is a rabbit polyclonal antibody that detects SCY1-Like Protein 1 and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-SCYL1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP, HT01 UniProt Number: Q96KG9
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ABS1494-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1494-25UG
DescriptionAnti-SCYL1
Alternate Names
  • N-terminal kinase-like protein
  • Coated vesicle-associated kinase of 90 kDa
  • SCY1-like protein 1
  • Telomerase regulation-associated protein
  • Telomerase transcriptional element-interacting factor
  • Teratoma-associated tyrosine kinase
Background InformationN-terminal kinase-like protein (UniProt: Q96KG9; also known as Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase) is encoded by the SCYL1 (also known as CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP, HT019) gene (Gene ID: 57410) in human. SCY1-like protein 1 is ubiquitously expressed and it regulates COPI-mediated retrograde protein traffic at the interface between the Golgi apparatus and the endoplasmic reticulum. It is involved in the maintenance of the Golgi apparatus morphology. It has a divergent N-terminal kinase domain (aa 14-314), but it is catalytically inactive. It also contain three HEAT repeats (aa 350-388; 389-427; and 507-545). Six isoforms of SCY1-like protein 1 have been reported that are produced by alternative splicing. Its intracellular distribution is isoform specific. Isoforms 1 and 2 are shown to be cytoplasmic throughout the cell cycle. Isoform 3 is cytoplasmic during interphase and centrosomal during mitosis. Isoform six is generally localized to nucleus and it acts as a transcriptional activator and binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. Mutations in SCYL1 gene are reported to cause Spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-SCYL1, Cat. No. ABS1494, is a rabbit polyclonal antibody that detects SCY1-Like Protein 1 and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SCYL1 in HeLa, A431, HUVEC, and NIH/3T3 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human SCY1-Like Protein 1.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects SCY1-Like Protein 1 (SCYL1) in human cells. It targets an epitope within 16 amino acids from the C-terminal region. The immunogen is conserved between isoforms 1, 2, and 3.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SCYL1
  • CVAK90
  • GKLP
  • NTKL
  • TAPK
  • TEIF
  • TRAP
  • HT01
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~100 kDa observed; 89.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected SCY1-Like Protein 1 (SCYL1) in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1494-25UG 04054839482113