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MABF3054-100UG Anti-SARS CoV-2 RBD mutant N501Y Antibody, clone 8A6

Anti-SARS CoV-2 RBD mutant N501Y clone 8A6, Cat. No. MABF3054, is a mouse monoclonal antibody that detects SARS-CoV-2 RBD mutant N501Y and is teste for use in ELISA and Western Blotting.

MABF3054-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMABF3054-100UG
DescriptionAnti-SARS CoV-2 RBD mutant N501Y Antibody, clone 8A6
Alternate Names
  • Spike glycoprotein Mutant N501Y
Background InformationSpike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Over the course of the pandemic, SARS-CoV2 has consistently mutated giving rise to various mutants. Clone 8A6 recognizes SARS-CoV-2 spike protein with N501Y mutation. Several variants, including B.2.2.7, B.1.351, and P.1 share this mutation on the receptor binding domain (RBD) and is a more infectious variant. RBD with N501Y mutation exhibits a stronger interaction with ACE2 receptor and slower dissociation rate. (Ref.: Liu, Y., et al. (2022). Nature. 602(7696); 294-299; Tian, F., et al. (2021). eLife. 10; e69091).
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SARS CoV-2 RBD mutant N501Y clone 8A6, Cat. No. MABF3054, is a mouse monoclonal antibody that detects SARS-CoV-2 RBD mutant N501Y and is teste for use in ELISA and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
Application NotesELISA Analysis: Serial dilutions of this antibody detected various recombinant SARS-CoV-2 protein samples

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide segment surrounding N501Y mutant site.
EpitopeExtracellular domain
Clone8A6
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 8A6 is a mouse monoclonal antibody that detects N501Y mutant form of of SARS-CoV-2 spike protein.
IsotypeIgGκ
Species Reactivity
  • Coronavirus
Species Reactivity NoteCoronavirus (SARS-CoV-2)
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • S
Modifications
  • Variant/Isoform
Purification MethodProtein G purified
UniProt Number
Molecular Weight115-130 kDa for recombinant SARS-CoV-2 Spike S1; 141.18 kDa calculated for full-length Spike protein.
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant SARS-CoV-2 Spike RBD (N501Y) protein .

Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant SARS-CoV-2 RBD mutant N501Y protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Global Trade Item Number
Catalogue Number GTIN
MABF3054-100UG 04065270845320