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MABF3162-25UL Anti-SARS-CoV-2 Antibody, RdRp NSP12 Antibody, clone 6H8-1

Anti-SARS-CoV-2, RdRp NSP12, clone 6H8-1, Cat. No. MABF3162, is a rat monoclonal antibody that detects SARS-CoV-2 RdRp (NSP12) and is tested for use in Immunocytochemistry and Western Blotting.

MABF3162-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF3162-25UL
DescriptionAnti-SARS-CoV-2 Antibody, RdRp NSP12 Antibody, clone 6H8-1
Alternate Names
  • Replicase polyprotein 1ab
  • EC: 2.7.7.48
  • RNA-directed RNA polymerase nsp12
  • Replicase polyprotein NSP12
  • Pol RdRp
Background InformationReplicase polyprotein 1ab (UniProt: P0DTD1: also known as pp1ab, EC: 2.7.7.48, RNA-directed RNA polymerase nsp12, Replicase polyprotein NSP12;Pol RdRp) is encoded by the rep (also known as 1a-1b) gene (Gene ID: 43740578) in SARS-CoV-2. SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. Its replicase polyprotein 1ab is cleaved into 16 different chains. Non-structural protein 12 (NSP12; aa 4393-5324) is the RNA-directed polymerase (RdRp) that is required for viral replication and transcription. NSP12 is shown to possesses low RNA polymerase activity and requires interaction with cofactors NSP7 and NSP8 to promote polymerase activity. Within the NSP12 is a right-hand RdRp domain (aa S367 to F920) and a nidovirus-specific N-terminal extension domain (aa D60 to R249) that adopts a nidovirus RdRp-associated nucleotidyltransferase (NiRAN) structural design. These two domains are connected by an interface domain (aa A250 to R365). The active site RdRp domain is formed by the conserved polymerase motifs A to G in the palm domain. Motif A that is composed of residues 611 to 626 (TPHLMGWDYPKCDRAM), contains the conserved classic divalent-cation-binding site D618, and motif C (aa 753 to 767 (FSMMILSDDAVVCFN)] contains the catalytic residues (aa 759 to 761). RdRp has become an important therapeutic target to treat COVID‐19. Inhibition of NSP12 activity not only blocks viral replication and reduces the systemic viral load but reduces pathogenesis associated with SARS‐CoV‐2 infection. (Ref.: Meng, W., et al. (2023). J. Med. Virol. 95(10; e28246; Machitani, M., et al. (2022). Virol. J. 19; 213; Gao, Y., et al. (2020). Science. 368(6492); 779-782; Hillen, HS., et al. (2020). Nature. 584(7819); 154-156).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV-2, RdRp NSP12, clone 6H8-1, Cat. No. MABF3162, is a rat monoclonal antibody that detects SARS-CoV-2 RdRp (NSP12) and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesTested applications

Immunocytochemistry Analysis: A representative lot detected SARS-CoV-2 RdRp (NSP12) in Immunocytochemistry application (Meng, W., et al. (2023). J Med Virol. 95(1):e28246).

Western Blotting Analysis: A representative lot detected SARS-CoV-2 RdRp (NSP12) in Western Blotting application (Meng, W., et al. (2023). J Med Virol. 95(1):e28246).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenA linear peptide corresponding to 19 amino acids from the NSP12 region of SARS-CoV-2 Replicase polyprotein 1ab.
EpitopeC-terminal half
Clone6H8-1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 6H8-1 is a rat monoclonal antibody that detects RNA-directed RNA polymerase NSP12 of the SARS-CoV-2.
IsotypeIgG2aκ
Species Reactivity
  • Virus
Species Reactivity NoteSARS-CoV-2
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • rep
Purification MethodProtein G purified
UniProt Number
Molecular Weight~100 kDa for recombinant RNA-directed RNA polymerase nsp12; 794.06 kDa for the entire replicase polyprotein. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant SARS-CoV-2 RdRp (NSP12) protein.

Western Blotting Analysis: A 1:250 dilution of this antibody detected recombinant SARS-CoV-2 RdRp (NSP12) protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3162-25UL 04065269366881