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NA18 Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19)

This Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19) is validated for use in Immunoblotting IF IP Paraffin Sections for the detection of Replication Protein A (Ab-2).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-RP-A

NA18
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ht, HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
NA18-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~34 kDa unphosphorylaed and the complexed (phosphorylated) subunit of replication protein A in HeLa and U293 cells and breast carcinoma tissue.
Catalogue NumberNA18
Brand Family Calbiochem®
SynonymsAnti-RP-A
References
ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Positive controlHeLa or U293 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5-5 µg/ml, see comments)
Application CommentsThis antibody can be used as a marker for proliferation. Will stain paraffin sections without pre-treatment, but pre-treating with trypsin or a pressure cooker enhances staining. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenreplication protein A purified from U293 cells
ImmunogenHuman
EpitopeWithin the p34 subunit of replication protein A
CloneRPA34-19
HostMouse
IsotypeIgG2a
Species Reactivity
  • Hamster
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NA18-100UG 04055977227680

Documentation

Anti-Replication Protein A (Ab-2) Mouse mAb (RPA34-19) Certificates of Analysis

TitleLot Number
NA18

References

Reference overview
Din, S., et al. 1990. Genes Dev. 4, 968.
Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
SynonymsAnti-RP-A
ApplicationImmunoblotting (5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/reaction)
Paraffin Sections (2.5-5 µg/ml, see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~34 kDa phosphorylated subunit and the nonphosphorylated RPA complex.
BackgroundThe replication system of a cell contains several essential components among which are replication factor A (RFA, RPA, or HeLa cell SSB), PCNA, and replication factor C (RFC) as well as several other proteins. RPA, a multi-subunit protein containing a 70 kDa, 34 kDa, and 11 kDa protein, may play a critical regulatory role in replication based on its functioning during both the initiation and elongation stages of DNA replication. The protein is highly conserved, having been identified in a range of species from yeast (Saccharomyces cerevisiae) to humans. The primary function of RPA appears to be as an auxiliary protein for both DNA polymerase α and δ. RPA undergoes a cell cycle dependent phosphorylation, which is restricted to the p34 protein. In addition to existing as part of the multi-subunit structure, p34 has also been identified as being present in a free, unphosphorylated form. Recent data shows that RPA is restricted to the nucleus and is overexpressed in transformed cells as well as in several tumor cells.
HostMouse
Immunogen speciesHuman
Immunogenreplication protein A purified from U293 cells
EpitopeWithin the p34 subunit of replication protein A
CloneRPA34-19
IsotypeIgG2a
Specieshamster, human
Positive controlHeLa or U293 cells or breast carcinoma tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody can be used as a marker for proliferation. Will stain paraffin sections without pre-treatment, but pre-treating with trypsin or a pressure cooker enhances staining. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesDin, S., et al. 1990. Genes Dev. 4, 968.
Brill, S.J. and Stillman, B. 1989. Nature 342, 92.
Stillman, B. 1989. Annu. Rev. Cell. Biol. 5, 197.
Tsurimoto, T. and Stillman, B. 1989. EMBO J. 8, 3883.
Wobbe, C.R., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 1834.