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PC130 Anti-Rad51 (Ab-1) Rabbit pAb

This Anti-Rad51 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Rad51 (Ab-1).

Anti-Rad51 (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC130
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC130-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the ~43 kDa Rad51 protein in ovarian tissue.
Catalogue NumberPC130
Brand Family Calbiochem®
Application Data
Detection of human RAD51 by immunoblotting. Sample: Whole cell lysate(10 µg) from HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

Detection of human RAD51 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HeLa cells. Antibody for IP: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (40 µl). Antibody for IB: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

Detection of human RAD51 by immunocytochemistry. Samples: A431 and HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:500). Detection: fluorescence (red) with DAPI counterstain (blue). Actin filaments were stained with Alexa Fluor® 488 dye -Phalloidin (green).
References
ReferencesBrendel, V., et al. 1997. J. Mol. Evol. 44, 528.
Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
Sharan, S.K., et al. 1997. Nature 386, 304.
Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
Tashiro, S., et al. 1996. Oncogene 12, 2165.
Ashley, T., et al. 1995. Chromosoma 104, 19.
Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4
Positive controlA431 cells, HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (1:2500)
Immunoprecipitation (40 µl/500µg total protein from lysate)
Immunocytochemistry (1:500)
Application CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.
Biological Information
Immunogenrecombinant, human Rad51
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC130-100UL 07790788053949

Documentation

Anti-Rad51 (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC130

References

Reference overview
Brendel, V., et al. 1997. J. Mol. Evol. 44, 528.
Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
Sharan, S.K., et al. 1997. Nature 386, 304.
Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
Tashiro, S., et al. 1996. Oncogene 12, 2165.
Ashley, T., et al. 1995. Chromosoma 104, 19.
Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.

Citations

Title
Viera et al. 2009. J. cell Science 122, 2149.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-June-2015 JSW
ApplicationImmunoblotting (1:2500)
Immunoprecipitation (40 µl/500µg total protein from lysate)
Immunocytochemistry (1:500)
Application Data
Detection of human RAD51 by immunoblotting. Sample: Whole cell lysate(10 µg) from HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

Detection of human RAD51 by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HeLa cells. Antibody for IP: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (40 µl). Antibody for IB: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:2500). Detection: chemiluminescence.

Detection of human RAD51 by immunocytochemistry. Samples: A431 and HeLa cells. Primary antibody: Anti-RAD51 Rabbit pAb (Cat. No. PC130) (1:500). Detection: fluorescence (red) with DAPI counterstain (blue). Actin filaments were stained with Alexa Fluor® 488 dye -Phalloidin (green).
DescriptionAffinity purified rabbit polyclonal antibody. Recognizes the ~43 kDa Rad51 protein.
BackgroundThe bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair. The human RAD51 protein is the homologue of these two proteins. While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA. Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus. Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature. This focal distribution may be blocked by inhibitors of transcription. RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change. RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.
HostRabbit
Immunogen speciesHuman
Immunogenrecombinant, human Rad51
IsotypeIgG
Specieshuman, mouse, rat
Positive controlA431 cells, HeLa cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4
Preservative≤0.1% sodium azide
CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBrendel, V., et al. 1997. J. Mol. Evol. 44, 528.
Boulikas, T., et al. 1997. Anticancer Res. 17, 843.
Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 463.
Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
Sharan, S.K., et al. 1997. Nature 386, 304.
Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10222.
Shen, Z., et al. 1996. J. Biol. Chem. 271, 148.
Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.
Tashiro, S., et al. 1996. Oncogene 12, 2165.
Ashley, T., et al. 1995. Chromosoma 104, 19.
Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2298.
CitationViera et al. 2009. J. cell Science 122, 2149.