Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

05-623-Z Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free

Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free is a highly specific mouse monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting, ICC & ChIP-seq.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: POLR2A, POLR2 UniProt Number: P24928
View Products on Sigmaaldrich.com
05-623-Z
200 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, YeastWB, ICC, ChIP-seqMPurifiedMonoclonal Antibody
Description
Catalogue Number05-623-Z
DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free
Alternate Names
  • DNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II subunit B1
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase III largest subunit
  • RNA-directed RNA polymerase II subunit RPB1
Background InformationRNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. (This product is Ascites Free.)
Quality LevelMQ100
Applications
ApplicationAnti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free is a highly specific mouse monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting, ICC & ChIP-seq.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • ChIP-seq
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected RNA polymerase II in 10 µg of HeLa cell lysate.

Immunocytochemistry Analysis: A 1:50 and 1:250 dilution from a representative lot detected RNA polymerase II in HeLa and A431 cells, respectively.
Biological Information
ImmunogenLinear peptide corresponding to human RNA polymerase II.
CloneCTD4H8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Yeast
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLR2A
  • POLR2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~220 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RNA polymerase II in 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-623-Z 04053252932656

Documentation

Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free SDS

Title

Safety Data Sheet (SDS) 

Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free Certificates of Analysis

TitleLot Number
Anti-RNA polymerase II, clone -2707237 2707237
Anti-RNA polymerase II, clone -2739008 2739008
Anti-RNA polymerase II, clone CTD4H8, Ascites Free - 3286043 3286043
Anti-RNA polymerase II, clone CTD4H8, Ascites Free - 3386476 3386476
Anti-RNA polymerase II, clone CTD4H8, Ascites Free - 3462124 3462124
Anti-RNA polymerase II, clone CTD4H8, Ascites Free - 3506610 3506610
Anti-RNA polymerase II, clone CTD4H8, Ascites Free - 3754137 3754137
Anti-RNA polymerase II, clone CTD4H8, Ascites Free - 4114805 4114805
Anti-RNA polymerase II, clone CTD4H8, Ascites Free - Q2299332 Q2299332
Anti-RNA polymerase II, clone CTD4H8, Ascites Free -2527083 2527083
Show more

Related Products & Applications

Related Products

Catalogue Number Description
04-1569 Anti-RNA polymerase II subunit B1 Antibody, clone 4F8
04-1570 Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12
04-1570-I Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12
04-1571 Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10
04-1571-I Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E10 (rat monoclonal).
04-1572 Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
04-1572-I Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
05-623 Anti-RNA polymerase II Antibody, clone CTD4H8
05-623-AF488 Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate
05-623-AF647 Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate
Show more

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies