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05-952-I-25UG Anti-RNA Polymerase II Antibody, CTD Antibody, clone 8WG16

Anti-RNA Polymerase II, CTD, clone 8WG16, Cat. No. 05-952-I, is a mouse monoclonal antibody that detects DNA-directed RNA polymerase II subunit RPB1 and has been tested for use in Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Inhibition assay, and Western Blotting.

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Research Category: Epigenetics & Nuclear Function Gene Symbol: POLR2A, POLR2 UniProt Number: P24928
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05-952-I-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, YeastDB, IP, Inhibition, WB, ChIPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-952-I-25UG
DescriptionAnti-RNA Polymerase II Antibody, CTD Antibody, clone 8WG16
Alternate Names
  • DNA-directed RNA polymerase II subunit RPB1
  • EC: 2.7.7.6
  • RNA polymerase II subunit B1
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase III largest subunit
  • RNA-directed RNA polymerase II subunit RPB1
Background InformationDNA-directed RNA polymerase II subunit RPB1 (UniProt: P24928; also known as EC: 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, is encoded by the POLR2A (also known as POLR2) gene (Gene ID: 5430) in human. DNA-dependent RNA polymerase II catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RPB1 subunit is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that suggested to grab the incoming DNA template. It has multiple zinc and magnesium ion binding sites and contains a repetitive C-terminal domain (CTD; aa 1593-1960) that consists of tandem repeats of the consensus sequence YSPTSPS and serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. It can exist in both hypophosphorylated form, which is mainly cytosolic, and in hyperphosphorylated form. The phosphorylation of Serine 2 and 5 in the hepatapeptide repeat is achieved by Cdk7 and Cdk9. Cdk7 phosphorylation promotes transcription initiation by triggering promoter release and elongation.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal antibody IgG2a in PBS without preservatives.
Applications
ApplicationAnti-RNA Polymerase II, CTD, clone 8WG16, Cat. No. 05-952-I, is a mouse monoclonal antibody that detects DNA-directed RNA polymerase II subunit RPB1 and has been tested for use in Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Inhibition assay, and Western Blotting.
Key Applications
  • Dot Blot
  • Immunoprecipitation
  • Inhibition
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
Application NotesWestern Blotting Analysis: A representative lot detected RNA Polymerase II, CTD in Western Blotting applications (Patturajan, M., et. al. (1998). J Biol Chem. 273(8):4689-94; Zhang, J., et. al. (1991). J Biol Chem. 266(4):2290-6).

Dot Blot Analysis: A representative lot detected RNA Polymerase II, CTD in Dot Blot applications (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).

Immunoprecipitation Analysis: A representative immunoprecipitated RNA Polymerase II, CTD in Immunoprecipitation applications (Bhaumik, S.R., et. al. (2001). Genes Dev. 15(15):1935-45).

Inhibition Analysis: A representative lot inhibited the interaction of RNA Polymerase II, CTD with the transcription complex. (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).
Biological Information
ImmunogenRNA Polymerase II from wheat germ extract
Clone8WG16
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 8WG16 specifically detects DNA-directed RNA polymerase II subunit RPB1.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Yeast
Species Reactivity NoteHuman, Yeast and almost all eukaryotic species, which has a concensus hepatapeptide repeat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLR2A
  • POLR2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~220 kDa observed; 217.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected RNA Polymerase II, CTD in HeLa cell nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-952-I-25UG 04054839563270