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MABE878 Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3

This Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 is validated for use in western blotting & ICC for the detection of RNA Helicase A/Dhx9.

Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Dhx9, Ddx9 UniProt Number: O70133
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MABE878
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE878
DescriptionAnti-RNA Helicase A/Dhx9 Antibody, clone 8E3
Alternate Names
  • ATP-dependent RNA helicase A
  • RHA
  • DEAH box protein 9
  • mHEL-5
  • Nuclear DNA helicase II
  • NDH II
Background InformationRNA Helicase A, also known as DEAH box protein 9 (mHEL-5), DHX9, or Nuclear DNA helicase II (NDH II), and encoded by the mouse gene Dhx9, is a component of the CRD-mediated mRNA stability complex. The CRD-mediated complex is a protein complex that binds to, and promotes stabilization of, mRNA molecules containing the coding region instability determinant (CRD). In humans, it consists of IGF2BP1 and at least four additional proteins: HNRNPU, SYNCRIP, YBX1, and RNA Helicase A. RNA Helicase A operates by unwinding dsDNA and RNA in a 3' to 5' direction. Additionally, RNA Helicase A acts as a transcriptional activator and interacts with LARP6 which is a protein that has been associated with fasting glucose traits, type 2 diabetes and obesity as well as the stabilization of collagen mRNAs for CO1A1 and CO1A2. RNA Helicase A is localized to the nucleolus within the nucleus but can also be found in the cytoplasm localized in cytoplasmic mRNP granules containing untranslated mRNAs and thus the protein shuttles between the nucleus and cytoplasm depending upon the microenvironment.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 is validated for use in western blotting & ICC for the detection of RNA Helicase A/Dhx9.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cell lysate (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).
Immunocytochemistry Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cells (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of mouse RNA Helicase A/Dhx9.
EpitopeN-terminus
Clone8E3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Dhx9
  • Ddx9
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~140 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RNA Helicase A/Dhx9 in 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE878 04053252946028

Documentation

Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 SDS

Title

Safety Data Sheet (SDS) 

Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 Certificates of Analysis

TitleLot Number
Anti-RNA Helicase A/Dhx9, clone 8E3 - Q2367874 Q2367874
Anti-RNA Helicase A/Dhx9, clone 8E3 - 3258758 3258758
Anti-RNA Helicase A/Dhx9, clone 8E3 - 3693962 3693962
Anti-RNA Helicase A/Dhx9, clone 8E3 -2790047 2790047
Anti-RNA Helicase A/Dhx9, clone 8E3 -2791165 2791165
Anti-RNA Helicase A/Dhx9, clone 8E3 -2821204 2821204
Anti-RNA Helicase A/Dhx9, clone 8E3 -2846345 2846345
Anti-RNA Helicase A/Dhx9, clone 8E3 -2888511 2888511
Anti-RNA Helicase A/Dhx9, clone 8E3 -2892484 2892484
Anti-RNA Helicase A/Dhx9, clone 8E3 Monoclonal Antibody 2930958

References

Reference overviewPub Med ID
Monoclonal antibody specific for Dhx9/NDHII/RHA.
Kotani, Manato, et al.
Hybridoma (Larchmt), 29: 259-61 (2010)  2010

Show Abstract 		20569003 20569003