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MABE1104-25UL Anti-RAG-2 Antibody, clone 39

Detect V(D)J recombination-activating protein 2 using this rabbit monoclonal Anti-RAG-2, clone 39, Cat. No. MABE1104-25UL, validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP), Immunofluorescence and Immunoprecipitation.

Research Category: Apoptosis & Cancer Gene Symbol: Rag2, Rag-2 UniProt Number: P21784
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MABE1104-25UL
25 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, ChIP, IF, IPRbCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABE1104-25UL
DescriptionAnti-RAG-2 Antibody, clone 39
Alternate Names
  • V(D)J recombination-activating protein 2
  • RAG-2
Background InformationV(D)J recombination-activating protein 2 (UniProt: P21784; also known as RAG-2) is encoded by the Rag2 (also known as Rag-2) gene (Gene ID: 19374) in murine species. RAG-2 is a nuclear protein that is a core component of the multiprotein RAG complex. It is predominantly found in maturing lymphoid cells. RAG complex mediates the DNA cleavage phase during V(D)J recombination. During this phase V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex is reported to occur by first introducing a nick in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct trans-esterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure is shown to play a critical role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at Lys4 (H3K4me3) stimulates both the nicking and hairpinning steps. RAG-2 contains an atypical PHD-type zinc finger (a 416-484) that recognizes and binds histone H3 trimethylated on Lys4 (H3K4me3). The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Although RAG-2 is not the catalytic component, it is required for all known catalytic activities mediated by RAG1. Mice with defective Rag2 gene fail to produce mature B or T-lymphocytes.
References
Product Information
FormatCulture Supernatant
PresentationRabbit monoclonal antibody in tissue culture supernatant without preservatives.
Quality LevelMQ100
Applications
ApplicationDetect V(D)J recombination-activating protein 2 using this rabbit monoclonal Anti-RAG-2, clone 39, Cat. No. MABE1104-25UL, validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP), Immunofluorescence and Immunoprecipitation.
Key Applications
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
  • Immunofluorescence
  • Immunoprecipitation
Application NotesImmunofluorescence Analysis: A representative lot detected RAG-2 in Immunofluorescence applications (Chan, E.A., et. al. (2013). Proc Natl Acad Sci USA. 110(48):E4628-37; Leu, T.M., et. al. (1995). Mol Cell Biol. 15(10):5657-70).

Immunoprecipitation Analysis: A representative lot detected RAG-2 in Immunoprecipitation applications (Leu, T.M., et. al. (1995). Mol Cell Biol. 15(10):5657-70).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected RAG-2 in Chromatin Immunoprecipitation applications (Dose, M., et. al. (2014). Proc Natl Acad Sci USA. 111(1):391-6; Ji, Y., et. al. (2010). Cell. 141(3):419-31).

Western Blotting Analysis: A representative lot detected RAG-2 in Western Blotting applications (Coster, G., et. al. (2012). J Biol Chem. 287(43):36488-98; Leu, T.M., et. al. (1995). Mol Cell Biol. 15(10):5657-70; Dose, M., et. al. (2014). Proc Natl Acad Sci USA. 111(1):391-6).
Biological Information
ImmunogenGST-tagged recombinantn fragment corresponind to the first 490 amino acids of murine V(D)J recombination-activating protein 2 (RAG-2).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityClone 39 is a rabbit monoclonal antibody that detects RAG-2 protein in murine species.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Rag2
  • Rag-2
Purification MethodUnpurified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse bone marrow tissue lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected RAG-2 in 10 µg of mouse bone marrow tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1104-25UL 04054839437854

Documentation