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MABE343-AF488 Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate Antibody

This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair
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MABE343-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMABE343-AF488
DescriptionAnti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate Antibody
Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE343.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenPuromycin-incorporated neosynthesized proteins at multiple molecular weights
Clone12D10
Concentration0.5 mg/mL
HostMouse
SpecificityThis antibody detects Puromycin-incorporated neosynthesized proteins.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightRefer to Cat. No. MABE343 for observed molecular weight information.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE343-AF488 04053252973918

Documentation

Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate Antibody Certificates of Analysis

TitleLot Number
Anti-Puromycin, clone 12D10, Alexa -2807044 2807044
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - Q2425065 Q2425065
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - 3174824 3174824
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - 3224587 3224587
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - 3275533 3275533
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - 3385862 3385862
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - 3494037 3494037
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - 3605504 3605504
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - 3734852 3734852
Anti-Puromycin, clone 12D10, Alexa Fluor® 488 Conjugate - 3739680 3739680
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies