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AB5200-200UL Anti-Potassium Channel GIRK2 Antibody

Anti-Potassium Channel GIRK2 Antibody detects level of Potassium Channel GIRK2 & has been published & validated for use in IH & WB.

Anti-Potassium Channel GIRK2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: KCNJ6, KATP-2, KCNJ7, MGC126596, BIR1, KATP2, hiGIRK2, KIR3.2, Kir3.2, GIRK2 UniProt Number: P48051
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AB5200-200UL
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5200-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Potassium Channel GIRK2 Antibody
Alternate Names
  • G-protein Activated Inwardly Rectifying Potassium Channel
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot use 20 ng of protein per minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationAnti-Potassium Channel GIRK2 Antibody detects level of Potassium Channel GIRK2 & has been published & validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenGST fusion protein from the C-terminal portion of mouse GIRK2 protein (amino acids 374-414) (Accession P48542).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes a full length Kir3.2 (GIRK2, G-protein Activated Inwardly Rectifying Potassium Channel 2). Does not cross react with any other potassium channel antigens tested so far.
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryPotassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and may be involved in the regulation of insulin secretion by glucose. It associates with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex.
Gene Symbol
  • KCNJ6
  • KATP-2
  • KCNJ7
  • MGC126596
  • BIR1
  • KATP2
  • hiGIRK2
  • KIR3.2
  • Kir3.2
  • GIRK2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P48051 # This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
SIZE: 423 amino acids; 48451 Da
SUBUNIT: Associates with GIRK1 or GIRK4 to form a G-protein- activated heteromultimer pore-forming unit. The resulting inward current is much larger (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.
SIMILARITY: SwissProt: P48051 ## Belongs to the inward rectifier-type potassium channel family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5200-200UL 04053252365843

Documentation

Anti-Potassium Channel GIRK2 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Potassium Channel GIRK2 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-Kir3.2 (GIRK2) - 3403765 3403765
RABBIT ANTI-Kir3.2 (GIRK2) - 3528150 3528150
RABBIT ANTI-Kir3.2 (GIRK2) - 3589832 3589832
RABBIT ANTI-Kir3.2 (GIRK2) -2557541 2557541
RABBIT ANTI-Kir3.2 (GIRK2) -2576330 2576330
RABBIT ANTI-Kir3.2 (GIRK2) -2586508 2586508
RABBIT ANTI-Kir3.2 (GIRK2) -2792520 2792520
RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY 2477831
RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY 3028352
RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY 3109235
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Data Sheet

Title
RABBIT ANTI-Kir3.2 (GIRK2) AFFINITY PURIFIED POLYCLONAL ANTIBODY