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16-199 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate

Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate detects level of Phosphotyrosine & has been published & validated for use in IP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: General Post-translation Modification
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16-199
1 mg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIPMGel ImmobilizedMonoclonal Antibody
Description
Catalogue Number16-199
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate
Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
References
Product Information
FormatGel Immobilized
Control
  • Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
PresentationGel Immobilized mouse monoclonal antibody covalently coupled to protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.01% Kathon, as a preservative for a total volume of 2 mL. Liquid suspension.
Quality LevelMQ100
Applications
ApplicationAnti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate detects level of Phosphotyrosine & has been published & validated for use in IP.
Key Applications
  • Immunoprecipitation
Application NotesThis product is useful for immunoprecipitation and immunoaffinity chromatography methods.
Biological Information
EpitopeN/A
Clone4G10®
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes tyrosine-phosphorylated proteins from all species.
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein A Purfied
Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

Immunoprecipitation Analysis:
10 μL of this lot immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8ºC from date of receipt.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
16-199 04053252735387

Documentation

Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate Certificates of Analysis

TitleLot Number
Anti-Phosphotyrosine, recombinant -2638024 2638024
Anti-Phosphotyrosine, recombinant clone 4G10#174;, agarose conjugate - DAM1631998 DAM1631998
Anti-Phosphotyrosine, recombinant clone 4G10#174;, agarose conjugate - DAM1731744 DAM1731744
Anti-Phosphotyrosine, recombinant clone 4G10#174;, agarose conjugate - DAM1813280 DAM1813280
Anti-Phosphotyrosine, recombinant clone 4G10#174;, agarose conjugate - JBC1381998 JBC1381998
Anti-Phosphotyrosine, recombinant clone 4G10reg;, agarose conjugate - 0609040558 0609040558
Anti-Phosphotyrosine, recombinant clone 4G10reg;, agarose conjugate - 22463 22463
Anti-Phosphotyrosine, recombinant clone 4G10®, agarose conjugate - 2420446 2420446
Anti-Phosphotyrosine, recombinant clone 4G10®, agarose conjugate - 2280865 2280865
Anti-Phosphotyrosine, recombinant clone 4G10®, agarose conjugate - 3175664 3175664
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Alternative Format

Catalogue Number Description
05-321 Anti-Phosphotyrosine Antibody, clone 4G10®
05-321MG Anti-Phosphotyrosine Antibody, clone 4G10®
05-321X Anti-Phosphotyrosine Antibody, clone 4G10®
05-777 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®
16-101 Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
16-103 Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate
16-104 Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate
16-105 Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate
16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
16-205 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, FITC conjugate

Product Families

Phosphotyrosine Signaling Solutions

Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more.
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Phosphotyrosine Antibodies

Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies