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07-330-AF488 Anti-Partitioning-defective 3 Antibody, Alexa Fluor® 488 Conjugate

This Anti-Partitioning-defective 3 Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Partitioning-defective 3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: PARD3, PAR3, PAR3A UniProt Number: Q8TEW0
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07-330-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, Mk, Ca, FgICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue Number07-330-AF488
DescriptionAnti-Partitioning-defective 3 Antibody, Alexa Fluor® 488 Conjugate
Alternate Names
  • Partitioning defective 3 homolog
  • PAR-3
  • PARD-3
  • Atypical PKC isotype-specific-interacting protein
  • ASIP
  • CTCL tumor antigen se2-5
  • PAR3-alpha
  • Partitioning-defective 3, Alexa Fluor® 488 Conjugate
Background InformationPartitioning defective 3 homolog (UniProt Q8TEW0; also known as ASIP, Atypical PKC isotype-specific-interacting protein, Bazooka, CTCL tumor antigen se2-5, Par-3 family cell polarity regulator alpha, Par-3 partitioning defective 3 homolog, PAR3-alpha, Protein phosphatase 1 regulatory subunit 118) is encoded by the PARD3 (also known as Baz, PAR3, PAR3A, PARD3A, PPP1R118) gene (Gene ID 56288) in human. Par3 is a PDZ (PSD95/Dlg/ZO-1) domain-containing membrane scaffold adapter protein that plays an important role in epithelial cell tight junction (TJ) formation and apico-basal polarity maintenance. Together with PAR6, atypical protein kinase C (aPKC), and the Rho GTPase CDC42, Par3 forms a complex that regulates cytoskeleton re-organization, post-golgi vesicle trafficking, microtubule network polarization, and asymmetric cell division. PAR6 interacts directly with aPKC isoforms iota/lambda and zeta to form the complex core. Recruitment of GTP-bound CDC42 to the PAR6-aPKC core induces aPKC phosphorylation/activation by PAR6, and subsequent PAR3 binding. Another quaternary apical junctional complex (AJC), Willin–Par6-aPKC–Par3, has also been reported. In this case, the complex-associated aPKC functions to phosphorylate ROCK, resulting in ROCK dissociation from the AJC and thereby preventing excessive ROCK-dependent apical constriction.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Partitioning-defective 3 Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Partitioning-defective 3.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Partitioning-defective 3 in A431, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-330) is shown to be suitable also for Western blotting, immunocytochemistry, and immunohistochemistry applications.
Biological Information
ImmunogenGST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with all 11 spliced isofoms (UniProt Q8TEW0-1 to Q8TEW0-11).
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Monkey
  • Canine
  • Frog
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PARD3
  • PAR3
  • PAR3A
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~180, 150, 100 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HUVECs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Partitioning-defective 3 in HUVECs.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-330-AF488 04055977296525

Documentation

Anti-Partitioning-defective 3 Antibody, Alexa Fluor® 488 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Partitioning-defective 3 Antibody, Alexa Fluor® 488 Conjugate Certificates of Analysis

TitleLot Number
Anti-Partitioning-defective 3, Alexa Fluor® 488 Conjugate 2892483
Anti-Partitioning-defective 3, Alexa Fluor® 488 Conjugate - 3204215 3204215
Anti-Partitioning-defective 3, Alexa Fluor® 488 Conjugate - 3914293 3914293
Anti-Partitioning-defective 3, Alexa Fluor® 488 Conjugate -2888515 2888515
Anti-Partitioning-defective 3, Alexa Fluor® 488 Conjugate -Q2614591 Q2614591

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications