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MABS197 Sigma-AldrichAnti-PTP1B Antibody, clone FG6-1G

Anti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.

Anti-PTP1B Antibody, clone FG6-1G MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Brochures

Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTPN1, PTP1B UniProt Number: P18031

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABS197
Replaces	04-1140
Description	Anti-PTP1B Antibody, clone FG6-1G
Alternate Names	Tyrosine-protein phosphatase non-receptor type 1 Protein-tyrosine phosphatase 1B PTP-1B
Background Information	PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.

References

Product Information
Format	Purified
Control	TF-1 cell lysate
Presentation	Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Western Blot Analysis: A representative lot was used to detect PTP1B in Jurkat cell lysate. Immunoprecipitation Analysis: A representative lot from an independent laboratory detected PTP1B in A431 cell lysate (Gulati, P. et al. (2004). EMBO Rep. 5(8):812-817.).

Biological Information
Immunogen	Recombinant protein corresponding to the catalytic domain of human PTP1B.
Epitope	Catalytic domain
Clone	FG6-1G
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes the catalytic domain of PTP1B.
Isotype	IgG2aκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_002818
Entrez Gene Summary	The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
Gene Symbol	PTPN1 PTP1B
Purification Method	Protein G Purified
UniProt Number	P18031
UniProt Summary	FUNCTION: May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. SUBUNIT STRUCTURE: Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note: Interacts with EPHA3 at the cell membrane. POST-TRANSLATIONAL MODIFICATION: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50. SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.
Molecular Weight	~50 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in TF-1 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PTP1B in 10 µg of TF-1 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABS197	04053252689826

Documentation

Anti-PTP1B Antibody, clone FG6-1G SDS

Title
Safety Data Sheet (SDS)

Anti-PTP1B Antibody, clone FG6-1G Certificates of Analysis

Title	Lot Number
Anti-PTP1B, clone FG6-1G	2479551
Anti-PTP1B, clone FG6-1G - 2386525	2386525
Anti-PTP1B, clone FG6-1G - 2432520	2432520
Anti-PTP1B, clone FG6-1G - 2376686	2376686
Anti-PTP1B, clone FG6-1G - 3167072	3167072
Anti-PTP1B, clone FG6-1G - 3387625	3387625
Anti-PTP1B, clone FG6-1G - 3585812	3585812
Anti-PTP1B, clone FG6-1G - 3720623	3720623
Anti-PTP1B, clone FG6-1G - 3901423	3901423
Anti-PTP1B, clone FG6-1G - 4080485	4080485

References

Reference overview	Pub Med ID
UVA inactivates protein tyrosine phosphatases by calpain-mediated degradation. Gulati, Pawan, et al. EMBO Rep., 5: 812-7 (2004) 2004 Show Abstract UV irradiation causes inflammatory and proliferative cellular responses. We have proposed previously that these effects are, to a large extent, caused by the ligand-independent activation of several receptor tyrosine kinases due to the inactivation of their negative control elements, the protein tyrosine phosphatases (PTPs). We examined the mechanism of this inactivation and found that, in addition to reversible oxidation of PTPs, UV triggers a novel mechanism: induced degradation of PTPs by calpain, which requires both calpain activation and substrate PTP oxidative modification. This as yet unrecognized effect of UV is irreversible, occurs predominantly with UVA and UVB, the range of wavelengths in sunlight that reach the skin surface, and at physiologically relevant doses.	15247926

Reference overview

Pub Med ID

Brochure

Title
New Products: Volume 3, 2012

Catalogue Number	Description
MABS456	Anti-PTP1B Antibody (Oxidized), clone scFvs 45
AP124P	Goat Anti-Mouse IgG Antibody, Peroxidase Conjugated, H+L

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