Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC165L Anti-Opioid μ Receptor (384-398) Rabbit pAb

This Anti-Opioid µ Receptor (384-398) Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, IF, IP for the detection of Opioid µ Receptor (384-398).

Anti-Opioid μ Receptor (384-398) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
PC165L
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC165L-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the ~44-45 kDa opiod μ receptor in caudate putamen and the dorsal horn of the spinal cord.
Catalogue NumberPC165L
Brand Family Calbiochem®
References
ReferencesZaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol. 36, 379.
Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.
Kieffer, B.L. 1995. Cell. Mol. Neurobiol. 15, 615-635.
Childers, S.R. 1991. Life Sci. 48, 1991.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat caudate putamen or spinal cord (dorsal horn)
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Wang, X., et al. 2004. Neurosci. 129, 751. Immunoblotting, Immunofluorescence, Frozen Sections Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesFrozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP)
Immunoblotting (1:2000-1:2500; see application references)
Immunofluorescence (1:100-1:200)
Immunoprecipitation (see comments)
Application CommentsThe specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC165L-100UL 04055977209501

Documentation

Anti-Opioid μ Receptor (384-398) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Opioid μ Receptor (384-398) Rabbit pAb Certificates of Analysis

TitleLot Number
PC165L

References

Reference overview
Zaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol. 36, 379.
Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.
Kieffer, B.L. 1995. Cell. Mol. Neurobiol. 15, 615-635.
Childers, S.R. 1991. Life Sci. 48, 1991.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2008 RFH
ApplicationFrozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP)
Immunoblotting (1:2000-1:2500; see application references)
Immunofluorescence (1:100-1:200)
Immunoprecipitation (see comments)
DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~44-45 kDa opioid µ receptor protein.
BackgroundOpioid peptides are endogenous neuromodulators, which have been identified from brain, and play a major role in the nociceptive pathway by interacting with several membrane receptors, defined as µ, δ, and κ. These peptides are derived from the cleavage of precursor proteins to produce the β-endorphin, enkephalin, and dynorphin peptides. Leu-enkephalin, Met-enkephalin, and β-endorphin all bind the µ and δ receptors with high affinity, while β-endorphin also exhibits low affinity κ binding. Dynorphin A and dynorphin B both bind the κ receptor with high affinity. In addition to these endogenous peptides, a number of exogenous nonpeptide molecules known as alkaloids or opiates also interact with the receptors to mediate a number of biological events which include the modulation of pain, analgesia, behavior, locomotor activity, and neuroendocrine physiology. Although the δ opioid receptor subtype was the first subtype identified, all three receptor classes are G protein-coupled receptors which have been shown to inhibit adenylate cyclase, decrease the conductance of voltage-gated Ca2+ channels, or activate K+ channel current.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor
IsotypeIgG
Speciesrat
Positive controlRat caudate putamen or spinal cord (dorsal horn)
FormLyophilized
FormulationUndiluted serum.
PreservativeNone
CommentsThe specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C).
Toxicity Highly Toxic
ReferencesZaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol. 36, 379.
Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.
Kieffer, B.L. 1995. Cell. Mol. Neurobiol. 15, 615-635.
Childers, S.R. 1991. Life Sci. 48, 1991.
Application referencesImmunoblotting Wang, X., et al. 2004. Neurosci. 129, 751. Immunoblotting, Immunofluorescence, Frozen Sections Arvidsson, U., et al. 1995. J. Neurosci. 15, 3328.