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AB9566-I Anti-OTX2 Antibody

Anti-OTX2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: OTX2 UniProt Number: P32243
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AB9566-I
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB9566-I
DescriptionAnti-OTX2 Antibody
Alternate Names
  • OTX2
  • Homeobox protein OTX2
  • Orthodenticle homolog 2
Background InformationHomeobox protein OTX2 (UniProt P32243; also known as Orthodenticle homolog 2) is encoded by the OTX2 (also known as CPHD6, MCOPS5) gene (Gene ID 5015) in human. OTX2 is a paired-type homeobox transcription factor that controls the expression of target genes by binding to regulatory regions containing the YTAATNN motifs. Studies conducted in mice shows developmental stage-dependent expression pattern. OTX2 is widely expressed in the epiblast, but becomes progressively regionalised during gastrulation to an anterior domain of the ectoderm (anterior neuroectoderm, ANE) that contains the precursor cells of the prosencephalon and the mesencephalon of the embryonic brain. The precursor cells for the anterior visceral endoderm (AVE) fail to translocate from the distal site to the anterior region of the pre-gastrulation stage Otx2-knockout embryos. The absence of the AVE, in turn, causes the loss of the source of morphogenetic signals for inducing neural differentiation of the ANE. In addtion, OTX2 is shown to be essential in the anterior mesendoderm (AME) for the induction of anterior neural tissues and head formation. OTX2 is reported to mediate the transcriptions of Dkk1 and Lhx1, loss of Otx2 in the AME results in the downregulation of Dkk1 and Lhx1.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected OTX2 in human retina tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the internal region of human OTX2.
EpitopeInternal region
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with both spliced isoforms, as well as all five polymorphic forms of human OTX2 reported by UniProt (P32243). The immunogen sequence is about 78% conserved in OTX1, this polyclonal antibody may therefore recognize OTX1. However, OTX1 cross-reactivity has not been directly tested and confirmed. OTX2 is expressed earlier than OTX1 and therefore this antibody can be used to identify OTX2 during development.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Rhesus Macaque, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • OTX2
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~35 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Y79 human retinoblastoma cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected OTX2 in 10 µg of Y79 human retinoblastoma cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB9566-I 04055977311778