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ABN78B Anti-NeuN (rabbit) Antibody, Biotin Conjugate

Anti-NeuN (rabbit) Antibody, Biotin Conjugate detects level of NeuN (rabbit) & has been published & validated for use in IH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: NeuN
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ABN78B
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIHCRbBiotinPolyclonal Antibody
Description
Catalogue NumberABN78B
DescriptionAnti-NeuN (rabbit) Antibody, Biotin Conjugate
Alternate Names
  • NEUronal Nuclei
  • Neuronal Nuclei
Background InformationABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
References
Product Information
FormatBiotin
Control
  • Adult mouse brain tissue
PresentationPurified rabbit polyclonal conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationAnti-NeuN (rabbit) Antibody, Biotin Conjugate detects level of NeuN (rabbit) & has been published & validated for use in IH.
Key Applications
  • Immunohistochemistry
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to mouse NeuN.
EpitopeN-terminus
HostRabbit
SpecificityThis antibody recognizes NeuN at the N-terminus. The unconjugated version if this antibody (ABN78) has been shown to work in human and rat
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Gene Symbol
  • NeuN
Purification MethodAffinity Purfied
Molecular WeightThe unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa. An uncharacterized band appears at ~62 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in adult mouse brain tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN78B 04053252531453

Documentation

Anti-NeuN (rabbit) Antibody, Biotin Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-NeuN (rabbit) Antibody, Biotin Conjugate Certificates of Analysis

TitleLot Number
Anti-NeuN (rabbit polyclonal), Biotin -2812306 2812306
Anti-NeuN (rabbit polyclonal), Biotin Conjugate 2461566
Anti-NeuN (rabbit polyclonal), Biotin Conjugate 2838458
Anti-NeuN (rabbit polyclonal), Biotin Conjugate 3005309
Anti-NeuN (rabbit polyclonal), Biotin Conjugate - 2051399 2051399
Anti-NeuN (rabbit polyclonal), Biotin Conjugate - 3242716 3242716
Anti-NeuN (rabbit polyclonal), Biotin Conjugate - 3792151 3792151
Anti-NeuN (rabbit polyclonal), Biotin Conjugate - 4093162 4093162
Anti-NeuN (rabbit polyclonal), Biotin Conjugate - NRG1922534 NRG1922534
Anti-NeuN (rabbit polyclonal), Biotin Conjugate -2668510 2668510