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MAB5384-I-25UG Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 132.38

Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38, Cat. No. MAB5384-I, is a highly specific mouse monoclonal antibody that targets Chondrin Sulfate Protoglycan 4 and has been tested for use in Immunofluorescence and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Cspg4, Ng2 UniProt Number: Q00657
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MAB5384-I-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, MWB, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5384-I-25UG
DescriptionAnti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 132.38
Alternate Names
  • Chondroitin sulfate proteoglycan 4
  • Chondroitin sulfate proteoglycan NG2
  • HSN tumor-specific antigen
  • NG2
Background InformationChondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form. (Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing PBS, pH 7.2, containing 0.05% sodium azide.
Applications
ApplicationAnti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38, Cat. No. MAB5384-I, is a highly specific mouse monoclonal antibody that targets Chondrin Sulfate Protoglycan 4 and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in Rat E16 brain tissuel lysate.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected NG2 Chondroitin Sulfate Proteoglycan in rat brain frozen sections, post-fixed in ethanol.
Biological Information
ImmunogenHEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222.
Clone132.38
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 132.38 is a mouse monoclonal antibody that detects Chondroitin sulfate proteoglycan 4 in rat brain tissue.
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Mouse
Species Reactivity NoteRat, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cspg4
  • Ng2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~260 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NG2 Chondroitin Sulfate Proteoglycan in NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB5384-I-25UG 04054839510250

Documentation

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 132.38 SDS

Title

Safety Data Sheet (SDS) 

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody, clone 132.38 Certificates of Analysis

TitleLot Number
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3381376 3381376
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3668178 3668178
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3884787 3884787
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3887829 3887829
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3910161 3910161
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 3940966 3940966
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 4027340 4027340
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 4104686 4104686
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 4146505 4146505
Anti-NG2 Chondroitin Sulfate Proteoglycan, clone 132.38 - 4187672 4187672
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