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MABT391 Anti-Myosin-10 Antibody, clone 5H4.1

Detect Myosin-10 using this Anti-Myosin-10 Antibody, clone 5H4.1 validated for use in western blotting.

Anti-Myosin-10 Antibody, clone 5H4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: MYH10 UniProt Number: P35580
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MABT391
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT391
DescriptionAnti-Myosin-10 Antibody, clone 5H4.1
Alternate Names
  • Myosin-10
  • Cellular myosin heavy chain, type B
  • Myosin heavy chain 10
  • Myosin heavy chain, non-muscle IIb
  • Non-muscle myosin heavy chain B
  • NMMHC-B
  • Non-muscle myosin heavy chain Iib
  • NMMHC II-b
  • NMMHC-IIB
Background InformationMyosin-10, also known as Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Non-muscle myosin heavy chain B, NMMHC-B, Non-muscle myosin heavy chain IIb, or NMMHC-IIB, and encoded by the gene MYH10, is a myosin protein found in non-muscle cells. Often called cellular myosin, Myosin-10 appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. For instance Mysoin-10, during cell spreading, plays an important role in cytoskeleton reorganization, focal contacts and lamellipodial extension. Additionally, Myosin-10 is involved in many developmental and cellular functions including: axon guidance, retina development, cerebellar Purkinje cell layer development, exocytosis, embryonic development, mitotic cytokinesis, neuron migration, nuclear migration, regulation of cell shape, retina development, cardiac myofibril assembly, and ventricular cardiac muscle cell and adult heart development. Myosin-10 is localized to cell projections and lamellipodia. Myosin-10 is expressed in many cells and particularly in neurons in the cerebellum, spinal cord, and retina.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Myosin-10 using this Anti-Myosin-10 Antibody, clone 5H4.1 validated for use in western blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Myosin-10 in 10 µg of HUVEC cell lysate.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Myosin-10.
Clone5H4.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MYH10
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~230 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Myosin-10 in 10 µg of C2C12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT391 04053252970696