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MABF1991-25UG Anti-Ly9 (CD229) Antibody, clone Ly9.7.144

Anti-Ly9 (CD229), clone Ly9.7.144, Cat. No. MABF1991, is a mouse monoclonal antibody that detects T-lymphocyte surface antigen Ly-9 and has been tested for use as an Agonist and in Flow Cytometry, Immunofluorescence, Immunodepletion.

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MABF1991-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF1991-25UG
DescriptionAnti-Ly9 (CD229) Antibody, clone Ly9.7.144
Alternate Names
  • T-lymphocyte surface antigen Ly-9
  • Cell surface molecule Ly-9
  • Lymphocyte antigen 9
  • SLAM family member 3
  • SLAMF3
  • Signaling lymphocytic activation molecule 3
  • CD229
Background InformationT-lymphocyte surface antigen Ly-9 (UniProt: Q01965; also known as Cell surface molecule Ly-9, Lymphocyte antigen 9, SLAM family member 3, SLAMF3, Signaling lymphocytic activation molecule 3, CD229) is encoded by the Ly9 (also known as Ly-9) gene (Gene ID: 17085) in murine species. Ly-9 is a single-pass type I membrane protein that serves as a homophilic cell surface receptor present on all lymphocytes and is highly expressed on innate like lymphocytes. Its activity is controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Ly-9 plays a negative role in the signaling pathways required for innate-like lymphocyte (iNKT) development in the thymus. It also promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion. Ly-9 is synthesized with a signal peptide (aa 1-47) that is cleaved off in the mature form. The mature form has an extracellular domain (aa 48-453), a short transmembrane domain (aa 454-474), and a cytoplasmic domain (aa 475-654). The extracellular domain contains two Ig-like V type (aa 48-158 and 250-362) and two Ig-like C type (aa 159-243 and 353-453) domains. In addition, LY-9 has two immunoreceptor tyrosine-based switch motifs (ITSM) motifs (aa 599-604 and 623-628), which mediate the interaction with the SH2 domain of SH2D1A and SH2D1B. Ly-9 deficient mice are reported to develop normally, but exhibit a significant difference in the distribution of thymocyte subsets compared to wild types. This antibody (clone Ly9.7.144) is shown to reduce the number of iNKT cells in the spleen and also reduce the absolute number of both NKT1 and NKT2 cells. (Ref.: Sintes, J., et al. (2013). J. Immunol. 190(1); 21-25; Cuenca, M et al., (2018). Eur. J. Immunol. 48(1); 99 105).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Applications
ApplicationAnti-Ly9 (CD229), clone Ly9.7.144, Cat. No. MABF1991, is a mouse monoclonal antibody that detects T-lymphocyte surface antigen Ly-9 and has been tested for use as an Agonist and in Flow Cytometry, Immunofluorescence, Immunodepletion.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunodepletion
Application NotesFlow Cytometry Analysis: A representative lot detected Ly9 (CD229) in Flow Cytometry applications (Sintes, J., et. al. (2013). J Immunol. 190(1):21-6; Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37).

Agonist Analysis: A representative lot of this antibody induced a significant decrease in the production of IL-4 in wild-type mice. (Sintes, J., et. al. (2013). J Immunol. 190(1):21-6).

Immunodepletion Analysis: A representative lot of this antibody selectively depleted splenic MZ and B1 B cells in wild-type BALB/c mice. (Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37; Cuenca, M., et. al. (2018). Eur J Immunol. 48(1):99-105).


Immunofluorescence Analysis: A representative lot detected Ly9 (CD229) in Immunofluorescence applications (Cuenca, M., et. al. (2016). J Immunol. 196(2):726-37).
Biological Information
Immunogen300.19 mouse tumor cells transfected with full-length murine Ly9 protein.
CloneLy9.7.144
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Ly9.7.144 detects T-lymphocyte surface antigen Ly-9 in murine cells. It targets an epitope with in the N-terminal Ig domain.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ly9
  • Ly-9
Purification MethodProtein G purified
UniProt Number
Molecular Weight73.14 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Mouse splenocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected Ly9 (CD229) in one million Mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1991-25UG 04054839646805