Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF1483 Anti-Ly-6G (mouse), violetFluor™ 450, clone RB6-8C5 Antibody

Anti-Ly-6G (mouse), violetFluor™ 450, clone RB6-8C5 Antibody is an antibody against Ly-6G for use in Flow Cytometry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Ly6g UniProt Number: P35461
View Products on Sigmaaldrich.com
MABF1483
25 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFCRvioletFluor™ 450Monoclonal Antibody
Description
Catalogue NumberMABF1483
DescriptionAnti-Ly-6G (mouse), violetFluor™ 450, clone RB6-8C5 Antibody
Alternate Names
  • Lymphocyte antigen 6G (Ly-6G)
  • Ly-6G.1
  • Ly-6G
Background InformationThe RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
References
Product Information
FormatvioletFluor™ 450
PresentationPurified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality LevelMQ100
Applications
ApplicationAnti-Ly-6G (mouse), violetFluor™ 450, clone RB6-8C5 Antibody is an antibody against Ly-6G for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneRB6-8C5
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Ly6g
Purification MethodPurified by size-exclusion chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 μg from a representative lot detected Ly-6G in one million C57Bl/6 bone marrow cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1483 04055977292046