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MABF3091-25UG Anti-LMP1 Antibody, clone 1G6

This rat monoclonal Anti-LMP1, clone 1G6, Cat. No. MABF3091 is tested for use in Immunocytochemistry, Western Blotting, and Immunohistochemistry, for the detection of Latent membrane protein 1 (LMP1).

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25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF3091-25UG
DescriptionAnti-LMP1 Antibody, clone 1G6
Alternate Names
  • LMP-1
  • Protein p25
  • Protein p63
Background InformationLatent membrane protein 1 (LMP1) (UniProt: P03230) is encoded by the LMP1 (also known as BNLF1) gene (Gene ID: 3783750) in Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4). Latent membrane protein 1 (LMP1) is a viral oncoprotein encoded by the Epstein-Barr virus (EBV) and is known for its critical role in EBV-associated oncogenesis. LMP1 functions as a constitutively active mimic of the tumor necrosis factor receptor (TNFR) and can activate various signaling pathways, including NF- B, JNK, and PI3K/Akt. Through its ability to modulate these pathways, LMP1 can promote cell proliferation, inhibit apoptosis, and induce the expression of various cytokines and growth factors. Additionally, LMP1 contributes to the immortalization of infected B cells and is implicated in the pathogenesis of EBV-associated malignancies, including Hodgkin's lymphoma and nasopharyngeal carcinoma.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationThis rat monoclonal Anti-LMP1, clone 1G6, Cat. No. MABF3091 is tested for use in Immunocytochemistry, Western Blotting, and Immunohistochemistry, for the detection of Latent membrane protein 1 (LMP1).
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesTested Applications

Immunohistochemistry Analysis: A representative lot detected LMP1 in Immunohistochemistry applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88; Shair, K.H.Y., et al. (2007). PLoS Pathog. 3(11):e166).

Immunocytochemistry Analysis: A representative lot detected LMP1 in Immunocytochemistry applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88).

Western Blotting Analysis: A representative lot detected LMP1 in Western Blotting applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88; Thornburg, N.J., et al. (2006). Oncogene. 25(2):288-97; Hannigan, A., et al. (2010). Mol Cancer. 9:9:184; Rancan, C., et al. (2015). PLoS Pathog. 11(6):e1004906).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenWild type LMP1-TrpE-fusion protein from Epstein-Barr virus.
EpitopeC-terminal cytoplasmic domain
Clone1G6
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 1G6 is a rat monoclonal antibody that detects LMP1. It targets an epitope within the C-terminal cytoplasmic domain of the protein.
IsotypeIgG2a
Species Reactivity
  • Virus
Species Reactivity NoteVirus.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LMP1
Purification MethodProtein G purified
UniProt Number
Molecular WeightTarget molecular weight 41.98 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in B95-8 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected LMP1 in B95-8 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3091-25UG 04065271270091