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MABS1781-100UL Anti-LGR4 Antibody, clone 7E7

Anti-LGR4, clone 7E7, Cat. No. MABS1781, is a rat monoclonal antibody that detects Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4) and is tested for use in Immunohistochemistry and Western Blotting.

MABS1781-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS1781-100UL
DescriptionAnti-LGR4 Antibody, clone 7E7
Alternate Names
  • Leucine-rich repeat-containing G-protein coupled receptor 4
  • G-protein coupled receptor 4
Background InformationLeucine-rich repeat-containing G-protein coupled receptor 4 (UniProt: Q9BXB1; also known as G-protein coupled receptor 48, LGR4) is encoded by the LGR4 (also known as GPR48) gene (Gene ID: 55366) in human. LGR4 is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. LGR4 contains fifteen LRR repeats. It is expressed in multiple steroidogenic tissues. Its expression has been reported in basal and granular cells in the skin, ductal epithelial cells in the breast, spermatogonia and tubule cells in the testis, islet cells in the pancreas, and tumor cells in the majority of colon cancer cases. LGR4 functions mainly through Wnt/β-catenin signaling to regulate cell proliferation, differentiation, and adult stem cell homeostasis. It serves as a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins, it is shown to associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors. This triggers the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, it does not activate heterotrimeric G-proteins to transduce the signal. LGR4 also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. It is also implicated in the survival of stem cells in the gut. Haplotype insufficiency of LGR4 is linked to increased risk of squamous cell carcinoma of the skin and to reduced birth weight and electrolyte imbalance. Mutations in LGR4 gene are known to cause osteoporosis. Clone 5A3 is reported to detect LGR4 in human and mouse tissues. (Ref.: Yi, J., et al. (2013). PLoS ONE. 8(10); e78144; Li, Z., et al. 2015). Front. Endocrinol. 6; 131).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-LGR4, clone 7E7, Cat. No. MABS1781, is a rat monoclonal antibody that detects Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4) and is tested for use in Immunohistochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesTested Applications

Immunohistochemistry Applications: A representative lot detected LGR4 in Immunohistochemistry applications (Yi, J., et al. (2013). PLoS One. 8(10):e78144).

Western Blotting Analysis: A representative lot detected LGR4 in Western Blotting applications (Yi, J., et al. (2013). PLoS One. 8(10):e78144).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length human LGR4 in an expression vector.
EpitopeUnknown
Clone7E7
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 7E7 is a rat monoclonal antibody that detects Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR).
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LGR4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~120 kDa observed;104.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LGR4 in HT-29 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1781-100UL 04065267780528