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MABE1896-100UG Anti-JunB Antibody, clone 75-5

Anti-JunB, clone 75-5, Cat. No. MABE1896, is a mouse monoclonal antibody that detects JunB and is tested for use in Immunocytochemistry and Western Blotting.

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MABE1896-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABE1896-100UG
DescriptionAnti-JunB Antibody, clone 75-5
Alternate Names
  • Transcription factor jun-B
  • MyD21
Background InformationTranscription factor Jun-B (UniProt: P09450; also known as MyD21) is encoded by the Junb (also known as Jun-b) gene (Gene ID: 16477) in mouse. JunB is a member of the AP-1 transcription family that binds to the DNA sequence 5'-TGA[CG]TCA-3' and is involved in regulating gene activity following the primary growth factor response. It can bind to DNA either as a homodimer or as a heterodimer with another member of the Jun/Fos family. JunB plays an important role in proliferation, differentiation, and death. With respect to cell cycle control, JunB is reported to have a dual function. It can serve as a cell division inhibitor and a tumor suppressor or can promote cell division. It is reported to diminish cell proliferation via transcriptional activation of p16 (INK4a). Its expression is shown to be very low in quiescent cells but can be rapidly induced by mitogenic stimuli during the G0/G1 transition. It induces proliferation, survival, drug resistance in multiple myeloma (MM) cells and silencing of JunB is reported to block MM cell proliferation in a murine xenograft model. It also protects MM cells against dexamethosome and bortezomib-induced cell death. JunB is also reported to regulate epidermal stem cells and sebaceous glands through balancing proliferation and differentiation of progenitors and by suppressing lineage infidelity. (Ref.: Singh, K., et al. (2018). Nat. Commun. 9; Article 3425; Fan, F., et al (2017). Leukemia. 31(7); 1570-1581; Farras, R., et al. (2008). Mol. Cell. Biol. 28(12); 4173-4187; Passegue, E., and Wagner, EF. (2000). EMBO J. 19(12); 2969-2979).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-JunB, clone 75-5, Cat. No. MABE1896, is a mouse monoclonal antibody that detects JunB and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected JunB in Western Blotting applications (Passegue, E., et. al. (2000). EMBO J. 19(12):2969-79; Farras, R., et. al. (2008). Mol Cell Biol. 28(12):4173-87; Gomard, T., et. al. (2010). PLoS One. 5(3):e9585).

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected JunB in HeLa cell line.

Immunocytochemistry Analysis: A representative lot detected JunB in Immunocytochemistry applications (Bakiri, L., et. al. (2000). EMBO J. 19(9):2056-68).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment correspond to the first 75 amino acids from the N-terminal region of mouse JunB.
EpitopeN-terminus
Clone75-5
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 75-5 is a mouse monoclonal antibody that detects human JunB. It targets an epitope within the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman. Predicted to react with Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Junb
  • Jun-b
Purification MethodProtein G purified
UniProt Number
Molecular Weight~43 kDa observed; 35.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected JunB in HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1896-100UG 04065265872638