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MABF227 Sigma-AldrichAnti-Interferon Lambda 4, clone 4G1 Antibody

This Anti-Interferon Lambda 4 antibody, clone 4G1 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting for the detection of Interferon Lambda 4.

Anti-Interferon Lambda 4, clone 4G1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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References
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Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: IFNL4 UniProt Number: K9M1U5

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, NhP	ELISA, FC, ICC, IHC, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABF227
Description	Anti-Interferon Lambda 4, clone 4G1 Antibody
Alternate Names	Interferon lambda-4 IFN-lambda-4
Background Information	Interferon Lambda 4, also known as IFN-lambda-4, and encoded by the gene IFNL4, is an important cytokine that forms part of our innate immunity against viruses. Interferon lambda-4 activates the JAK-STAT signaling pathway and ultimately up regulates several interferon specific stimulated genes. Interferon lambda-4 expression can be stimulated by nucleotide strands particularly ployinosinic-polycytidylic acid (polyI:C) which mimics the double-stranded hepatitis C virus. A genetic variation in the Interferon lambda-4 gene is associated with susceptibility to HCV infection. The mutation of a single base insertion results in gene inactivation resulting in defective immune response to HCV. Interferon lambda-4 signaling operates through Interferon lambda R1 and IL-10R2 receptors and the mutation is associated with defective glycosylation which is required for secretion, thus the amplification of the signal becomes compromised resulting in susceptibility to HCV as well as coronaviruses.

References

Product Information
Format	Purified
Presentation	Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Interferon Lambda 4 antibody, clone 4G1 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting for the detection of Interferon Lambda 4.
Key Applications	ELISA Flow Cytometry Immunocytochemistry Immunohistochemistry Western Blotting
Application Notes	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Interferon Lambda 4 in human and rat liver tissue sections. ELISA Analysis: A representative lot was conjugated with a sulfo-tag (MSD) and employed as the detection antibody in a sandwich ELISA application. An exogenously expressed IFNL4 HaloTag® fusion was found secreted in the culture media from IFNL4-Halo construct-transfected, but not mock-transfected HepG2 cells (Onabajo, O.O., et al. (2015). J. Interferon. Cytokine Res. 35(11):888-900). Flow Cytometry Analysis: A representative lot immunostained HepG2 cells transfected to express HaloTag® fusion IFN-λ4 orthologs of elephant, human and non-human primate (chimpanzee, orangutan, rhesus, marmoset, cynomolgus) origins, but not cells expressing IFNL4 of bovine, bat, canine, panda, and porcine species (Paquin, A., et al. (2016). J. Interferon Cytokine Res. 36(1):30-36). Immunocytochemistry Analysis: Representative lots immunostained HepG2 cells transfected to express HaloTag® fusion of IFN-λ4 orthologs of elephant, human and non-human primate (chimpanzee, orangutan, rhesus, marmoset, cynomolgus) origins (Paquin, A., et al. (2016). J. Interferon Cytokine Res. 36(1):30-36; Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171). Immunocytochemistry Analysis: A representative lot detected a time-dependent induction of IFNL4 expression in hepatocytes from a donor heterozygous for ss469415590 (TT/ΔG) upon PolyI:C treatment or hepatitis C virus (HCV) strain JFH1HCV infection in cultures (Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171). Western Blotting Analysis: A representative lot detected HaloTag® fusion IFN-λ4 orthologs of elephant, human and non-human primate (chimpanzee, orangutan, rhesus, marmoset, cynomolgus) origins, but not IFNL4 of bovine, bat, canine, panda, and porcine species transiently expressed in HepG2 cells (Paquin, A., et al. (2016). J. Interferon Cytokine Res. 36(1):30-36). Western Blotting Analysis: A representative lot detected exogenously expressed IFNL4 HaloTag® fusion constructs in transfected COS-7 and HepG2 cells, but not in untransfected cells. Clone 4G1 does not cross-react with IFN-alpha or IL-28B (Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171).

Biological Information
Immunogen	Linear peptide corresponding to an internal sequence from the N-termninal half of human interferon lambda 4.
Epitope	Internal (N-terminal half).
Clone	4G1
Concentration	1 mg/mL
Host	Mouse
Specificity	Clone 4G1 detected IFNL4 expression induction in primary hepatocytes (e.g. by PolyIC or by HCV) and exogenously overexpressed IFNL4 in HepG2 cells. IFNL4 expression is not detectable in normal liver cells (Dr. Ludmila Prokunina-Olsson, National Cancer Institute, NIH, Bethesda, MD). Clone 4G1 does not cross-react with IFN-alpha or IL-28B (Prokunina-Olsson, L., et al. (2013). Nat. Genet. 45(2):164-171).
Isotype	IgG1κ
Species Reactivity	Human Non-human Primate
Species Reactivity Note	Human, Non-Human Primate. Not Bovine, Bat, Canine, Panda, or Porcine.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001263183
Gene Symbol	IFNL4
Purification Method	Protein G Purified
UniProt Number	K9M1U5
Molecular Weight	~21 kDa observed.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting of human IFNL4 recombinant protein. Western Blotting Analysis: 1.0 µg/mL of this antibody detected 0.1 µg of human IFNL4 recombinant protein.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABF227	04053252976339

Documentation

Anti-Interferon Lambda 4, clone 4G1 Antibody SDS

Title
Safety Data Sheet (SDS)

Anti-Interferon Lambda 4, clone 4G1 Antibody Certificates of Analysis

Title	Lot Number
Anti-Interferon Lambda 4, clone 4G1 - 3221521	3221521
Anti-Interferon Lambda 4, clone 4G1 - 3668157	3668157
Anti-Interferon Lambda 4, clone 4G1 - 3994022	3994022
Anti-Interferon Lambda 4, clone 4G1 -2523221	2523221
Anti-Interferon Lambda 4, clone 4G1 -2618511	2618511
Anti-Interferon Lambda 4, clone 4G1 -2668548	2668548
Anti-Interferon Lambda 4, clone 4G1 -2683306	2683306
Anti-Interferon Lambda 4, clone 4G1 -2733290	2733290
Anti-Interferon Lambda 4, clone 4G1 -2855616	2855616
Anti-Interferon Lambda 4, clone 4G1 Monoclonal Antibody	2930199

References

Reference overview	Pub Med ID
A variant upstream of IFNL3 (IL28B) creating a new interferon gene IFNL4 is associated with impaired clearance of hepatitis C virus. Prokunina-Olsson, Ludmila, et al. Nat. Genet., 45: 164-71 (2013) 2013 Show Abstract Chronic infection with hepatitis C virus (HCV) is a common cause of liver cirrhosis and cancer. We performed RNA sequencing in primary human hepatocytes activated with synthetic double-stranded RNA to mimic HCV infection. Upstream of IFNL3 (IL28B) on chromosome 19q13.13, we discovered a new transiently induced region that harbors a dinucleotide variant ss469415590 (TT or ΔG), which is in high linkage disequilibrium with rs12979860, a genetic marker strongly associated with HCV clearance. ss469415590[ΔG] is a frameshift variant that creates a novel gene, designated IFNL4, encoding the interferon-λ4 protein (IFNL4), which is moderately similar to IFNL3. Compared to rs12979860, ss469415590 is more strongly associated with HCV clearance in individuals of African ancestry, although it provides comparable information in Europeans and Asians. Transient overexpression of IFNL4 in a hepatoma cell line induced STAT1 and STAT2 phosphorylation and the expression of interferon-stimulated genes. Our findings provide new insights into the genetic regulation of HCV clearance and its clinical management.	23291588

Reference overview

Pub Med ID

Brochure

Title
New Products - Antibodies, Assays, Small Molecules, Inhibitors, and Proteins Volume 2, 2014

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