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06-429-I Anti-IGF-IRα subunit Antibody

This Anti-IGF-IRα subunit Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of IGF-IRα subunit.

Anti-IGF-IRα subunit Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: IGF1R UniProt Number: P08069
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06-429-I
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, ICCChAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-429-I
DescriptionAnti-IGF-IRα subunit Antibody
Alternate Names
  • Insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • IGF-I receptor
  • CD221
  • Insulin-like growth factor 1 receptor alpha chain
  • Insulin-like growth factor 1 receptor beta chain
  • IGF-IRα subunit
Background InformationInsulin-like growth factor 1 receptor (EC:2.7.10.1; UniProt P08069; also known as CD221, IGF-I receptor, Insulin-like growth factor I receptor) is encoded by the IGF1R (also known as CD221, IGFIR, IGFR, JTK13) gene (Gene ID 3480) in human. The insulin-like growth factor 1 (IGF-1) receptor has tyrosine kinase that is activated by IGF-1 and IGF-2 binding. IGF-1 receptor is initially produced as a precursor protein with an aignal peptide sequence (aa 1-30). Post-translational cleavages produce one extracellular alpha (aa 31-736) chain and one transmembrane beta chain (aa 741 – 1367) linked together by a disulfide bond. Two sets of alpha-beta dimers then form the functional tetrameric receptor complex via a disulfide linkage between their alpha chain. Upon ligand binding, the alpha chains induce the tyrosine auto-phosphorylation of the beta chains, which in turn triggers cell type-specific downstream signaling cascade of events.
References
Product Information
FormatAffinity Purified
PresentationPurified chicken polyclonal IgY antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-IGF-IRα subunit Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of IGF-IRα subunit.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesImmunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated human IGF-IRα subunit in 0.5 mg of human IGF-IR-expressing C4 cell lysate.
Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected IGF-IRα subunit in human IGF-IR-expressing C4 cells.
Biological Information
ImmunogenKLH-conjugated synthetic peptide [C-KYADGTIDIEEVTENPKT] corresponding to amino acid residues 672-689 of human IGF-IR alpha chain.
EpitopeThird Fibronectin Type III (FnIII 3) domain
ConcentrationPlease refer to lot specific datasheet.
HostChicken
IsotypeIgY
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • IGF1R
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~110/180 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected IGF-IRα subunit in 10 µg of human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
06-429-I 04055977182453

Documentation

Anti-IGF-IRα subunit Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-IGF-IRα subunit Antibody Certificates of Analysis

TitleLot Number
Anti-IGF-IRα subunit - 3268883 3268883
Anti-IGF-IRα subunit - 4045750 4045750
Anti-IGF-IRα subunit -Q2589621 Q2589621
Anti-IGF-IRα subunit -Q2589625 Q2589625

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

Posters

Title
Post-Translational Modifications

Related Products & Applications

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06-429 Anti-IGF-IRα subunit Antibody
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies