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05-1571 Sigma-AldrichAnti-IGF-1R Antibody, clone 24-57

This Anti-IGF-1R Antibody, clone 24-57 is validated for use in IH, IP for the detection of IGF-1R.

Anti-IGF-1R Antibody, clone 24-57 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Metabolism Research Sub-Category: Insulin/Energy Signaling Gene Symbol: IGF1R, CD221, IGFIR, IGFR, JTK13 UniProt Number: P08069

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	IHC, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-1571
Description	Anti-IGF-1R Antibody, clone 24-57
Alternate Names	CD221 antigen IGF-I receptor Insulin-like growth factor I receptor insulin-like growth factor 1 receptor soluble IGF1R variant 1 soluble IGF1R variant 2
Background Information	There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.

References

Product Information
Format	Purified
Control	Human placental villi and tonsil tissue
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-IGF-1R Antibody, clone 24-57 is validated for use in IH, IP for the detection of IGF-1R.
Key Applications	Immunohistochemistry Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Soos, M., et al. (1992). Journal of Biological Chemistry. 267(18):12955-12963.)

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human IGF-IR.
Epitope	Unknown
Clone	24-57
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes IGF-IR.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000866.1
Entrez Gene Summary	This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq].
Gene Symbol	IGF1R CD221 IGFIR IGFR JTK13
Purification Method	Protein G Purified
UniProt Number	P08069
UniProt Summary	FUNCTION: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. SIZE: 1367 amino acids; 154793 Da SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed in a variety of tissues. PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin- like growth factor I (IGF I). & Phosphorylation of Tyr-980 is required for IRS1- and SHC1- binding. DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a growth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 3 fibronectin type-III domains. & Contains 1 protein kinase domain.
Molecular Weight	155 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunohistochemistry in human placental villi and tonsil tissue. Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected IGF-1R in human placental villi and tonsil tissue.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
05-1571	04053252363559

Documentation

Anti-IGF-1R Antibody, clone 24-57 SDS

Title
Safety Data Sheet (SDS)

Anti-IGF-1R Antibody, clone 24-57 Certificates of Analysis

Title	Lot Number
Anti-IGF-1R, clone 24-57	2485684
Anti-IGF-1R, clone 24-57 - 2410231	2410231
Anti-IGF-1R, clone 24-57 - 2066717	2066717
Anti-IGF-1R, clone 24-57 - 2205005	2205005
Anti-IGF-1R, clone 24-57 - 3025881	3025881
Anti-IGF-1R, clone 24-57 - 3232421	3232421
Anti-IGF-1R, clone 24-57 - 3323028	3323028
Anti-IGF-1R, clone 24-57 - 4227707	4227707
Anti-IGF-1R, clone 24-57 - NG1838620	NG1838620
Anti-IGF-1R, clone 24-57 - NRG1697045	NRG1697045

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