Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABS1064-I-25UL Anti-Hypusine

Anti-Hypusine Antibody, Cat. No. ABS1064-I, is a rabbit polyclonal antibody that detects Hypusine and has been tested for use in Western Blotting.

View Products on Sigmaaldrich.com
ABS1064-I-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABS1064-I-25UL
DescriptionAnti-Hypusine
Alternate Names
  • N -4-amino-2-hydroxybutyl(lysine)
Background InformationHypusine (N -4-amino-2-hydroxybutyl(lysine)) is derived by a post-translational modification of lysine. It is found in all eukaryotes and in certain archaea, but not in eubacteria. Currently, eukaryotic translation initiation factor5A (eIF-5A) is the only protein that has been reported to contain hypusine. This modification of elF-5A involves two reactions where in the first reaction deoxyhypusine synthase (DHS) transfers the butylamine moiety of spermidine to the -amino group of a specific lysine residue of eIF-5A resulting in the formation of deoxy-hypusine. This is followed by a hydroxylation reaction catalyzed by deoxyhypusine hydroxylase. This modification appears to be irreversible. The hypusinated form of elF-5A is reported to be the active form that is involved in translational elongation, RNA binding, and ribosome association. Hypusinated eIF5A is shown to be essential for cellular proliferation and responses to extracellular stressors. In the absence of an active hypusine cell proliferation is inhibited and Inhibition of hypusine formation is shown to confer cellular survival in certain stress states, such as infections, carcinogenesis, and obesity. (Ref.: Park, MH., and Wolff, EC. (2018). J. Biol. Chem. 293(48) 18710-18718; Nishiki, Y., et al. (2013). Springerplus. 2;421; Zhai, Q., et al. (2016). J. Mol. Biol. 428(3);603-617).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.02% sodium azide.
Applications
ApplicationAnti-Hypusine Antibody, Cat. No. ABS1064-I, is a rabbit polyclonal antibody that detects Hypusine and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Hypusine in lysate from HEK293 cells transfected with GFP-elF5a wild-type. (Courtesy of Dr. Raghu G Mirmira at Indiana University).
Biological Information
ImmunogenSynthetic hypusine-containing peptide C-Ahx-STSKTG[hypusine]HGHAKV-amide.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects hypusinated form of elF-5A.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with all mammals.
Antibody TypePolyclonal Antibody
Purification MethodUnpurified
Molecular Weight~45 kDa observed (GFP-elf-5A). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells transfected with GFP-elF5a WT.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Hypusine in lysate from HEK293 cells transfected with GFP-elF5a wild-type.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1064-I-25UL 04061841235996