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MAB1794H Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated

Detect CD19 using this Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD19, B4, MGC12802, Leu-12 UniProt Number: P15391
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MAB1794H
100 assays  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPhycoerythrinMonoclonal Antibody
Description
Catalogue NumberMAB1794H
ReplacesCBL141P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated
OverviewBIOCHEMISTRY:

p I = 7.0
Alternate Names
  • B-lymphocyte Surface Antigen B4
References
Product Information
FormatPhycoerythrin
HS Code3002 15 90
PresentationPurified from mouse ascitic fluid. R-Phycoerythrin conjugated. The antibody is supplied in 1.0ml PBS 0.01M, Sodium Azide 0.1%, pH 7.1, 0.2% BSA. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Quality LevelMQ100
Applications
ApplicationDetect CD19 using this Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated validated for use in FC.
Key Applications
  • Flow Cytometry
Application NotesThis antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry.

SUGGESTED USAGE

Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E(6) peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
Biological Information
ImmunogenRaji cells
CloneFMC63
HostMouse
SpecificityThis specificity of this antibody is equivalent to that of CD19 monoclonal antibodies (Zola et al., 1988, 1989), based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma.

Cell reactivity:

Normal:Stains all B lymphocytes in peripheral blood and spleen. Negative on granulocytes, monocytes, platelets, erythrocytes and T lymphocytes.

Clinical:

B cell chronic lymphocytic leukemia (B-CLL) +

Pro lymphocytic leukemia (PLL +

Hairy cell leukemia (HCL) +

Common acute lymphoblastic leukemia (CALL) +

Pre-B acute lymphoblastic leukemia (pre-B-ALL) +

NULL-acute lymphoblastic leukemia (NULL-ALL) +

T-acute lymphoblastic leukemia (T-ALL) -

T-common lymphocytic leukemia (T-CLL -
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryLymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Gene Symbol
  • CD19
  • B4
  • MGC12802
  • Leu-12
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P15391 # Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
SIZE: 556 amino acids; 61128 Da
SUBUNIT: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE:SwissProt: P15391 # Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265].
SIMILARITY: SwissProt: P15391 ## Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Packaging Information
Material Size100 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1794H 04053252579516

Documentation

Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated SDS

Title

Safety Data Sheet (SDS) 

Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated Certificates of Analysis

TitleLot Number
Anti-CD19, Monoclonal Antibody R-PE Conjugate, Ready To Use -
MAB1794H - 08061500640 08061500640
Mouse Anti-Human B Cells (CD-19) R-Phycoerythrin conjugated, Ready to use - 3991464 3991464
Mouse anti HUMAN B CELLS (CD19) 2472940
Mouse anti HUMAN B CELLS (CD19) - 3212301 3212301
Mouse anti HUMAN B CELLS (CD19), Monoclonal Antibody R-PHYCOERYTHRIN* CONJUGATED, READY TO USE - 2377261 2377261
Mouse anti HUMAN B CELLS (CD19), Monoclonal Antibody - 2390553 2390553
Mouse anti HUMAN B CELLS (CD19), Monoclonal Antibody - 2562359 2562359
Mouse anti HUMAN B CELLS (CD19), Monoclonal Antibody - 3003738 3003738
Mouse anti HUMAN B CELLS (CD19), Monoclonal Antibody - 3148500 3148500
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