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MABF2323-25UG Anti-HLA-A02:01 EBV-EBNA1 Antibody, clone MAB13

Anti-HLA-A02:01 EBV-EBNA1, clone MAB13, Cat. No. MABF2323, is a mouse monoclonal antibody that detects EBNA1 and is used in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Surface Plasmon Resonance & Western Blotting.

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MABF2323-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF2323-25UG
DescriptionAnti-HLA-A02:01 EBV-EBNA1 Antibody, clone MAB13
Alternate Names
  • Epstein-Barr nuclear antigen 1
  • EBNA-1
  • EBV nuclear antigen 1
Background InformationEpstein-Barr nuclear antigen 1 (UniProt: P03211; also known as EBNA-1, EBV nuclear antigen 1) is encoded by the EBNA1 (also known as BKRF1) gene (Gene ID: 3783709) in Epstein-Barr virus (EBV). EBV is a gamma-herpesvirus, a causative agent of infectious mononucleosis. Although the majority of virus infected individuals remain healthy, EBV has been implicated in the pathogenesis of African Burkitt s lymphoma and nasopharyngeal carcinoma. EBNA1, a multi-functional protein, plays an important role in maintaining EBV in the host cell and has been implicated in host cell immortalization. It plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. It is also reported to direct the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes and is known to activate the transcription of several viral latency genes. By counteracting the stabilization of host p53, it also reduces apoptosis and increases host cell survival. EBNA1 is also a risk factor in a variety of malignancies and is the only viral protein found in all EBV-related malignancies. Its C-terminal region contains a dimerization domain and a DNA binding domain (aa 459-607). Although this domain is essential for its replication function of EBNA1, it is not sufficient for this activity and the N-terminal half of EBNA1 is also required.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-HLA-A02:01 EBV-EBNA1, clone MAB13, Cat. No. MABF2323, is a mouse monoclonal antibody that detects EBNA1 and is used in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Surface Plasmon Resonance & Western Blotting.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Surface plasmon resonance
  • Western Blotting
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in Immunofluorescence applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Sim, A.C.N., et al. (2013). Sci Rep. 3:3232).

Immunohistochemistry Applications: A representative lot detected HLA-A02:01 EBV-EBNA1 in Immunohistochemistry applications (Sim, A.C.N., et al. (2013). Sci Rep. 3:3232).

Western Blotting Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in Western Blotting applications (Sim, A.C.N., et al. (2013). Sci Rep. 3:3232).

Flow Cytometry Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in Flow Cytometry applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Lai, J., et al. (2017). Sci Rep. 7(1):9923).

Immunocytochemistry Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in Immunocytochemistry applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Lai, J., et al. (2017). Sci Rep. 7(1):9923).

Surface plasmon resonance: A representative lot detected HLA-A02:01 EBV-EBNA1 in Surface plasmon resonance applications (Sim, A.C.N., et al. (2013). Sci Rep. 3:3232).

ELISA Analysis: A representative lot detected HLA-A02:01 EBV-EBNA1 in ELISA applications (Lai, J., et al. (2016). Blood. 128(10):1396-407; Lai, J., et al. (2017). Sci Rep. 7(1):9923).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenMembrane free HLA-A201 with EBV (strain B95-8) EBNA1 (FMVFLQTHI) 562-570 recombinant protein.
EpitopeC-terminal
CloneMAB13 (also referenced as E1)
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone MAB13 (E1) is a mouse monoclonal antibody that detects EBNA1. It targets an epitope within 9 amino acids from the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • EBNA1
Purification MethodProtein G purified
UniProt Number
Molecular Weight56.43 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in T2 cells pulsed with EBNA1 peptide (562-570).

Flow Cytometry Analysis (FC): 1 µg of this antibody detected HLA-A2-EBV EBNA1 in one million T2 cells pulsed with EBNA1 peptide (562-570).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2323-25UG 04065267061542