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AB5808-200UL Anti-HCN4 Antibody

This Anti-HCN4 Antibody is validated for use in IH, WB for the detection of HCN4.

Anti-HCN4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: HCN4 UniProt Number: Q9Y3Q4
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AB5808-200UL
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5808-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-HCN4 Antibody
Alternate Names
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationThis Anti-HCN4 Antibody is validated for use in IH, WB for the detection of HCN4.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry using rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenGST fusion protein corresponding to amino acids 119-155 of human HCN4 (Accession XP_007730, NP_005468, CAB42604, CAB52754).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4). The epitope does not share homology with any other known proteins.
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HCN4
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9Y3Q4 # Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli.
SIZE: 1203 amino acids; 129042 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus.
DOMAIN:SwissProt: Q9Y3Q4 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
DISEASE: SwissProt: Q9Y3Q4 # Defects in HCN4 are a cause of autosomal dominant sick sinus syndrome (SSS) [MIM:163800]; also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors, in which case it is considered to be a congenital disorder.
SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
MISCELLANEOUS: Inhibited by extracellular cesium ions.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5808-200UL 04053252507878

Documentation

Anti-HCN4 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-HCN4 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2453133 2453133
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) - 3276120 3276120
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2938948
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 3074963
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2907669
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 3106566
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 3305202
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2001220 2001220
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2042715 2042715
RABBIT ANTI-HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 4) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2064273 2064273
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References

Reference overviewApplicationSpeciesPub Med ID
Targeted insertion of the Cre-recombinase gene at the phenylethanolamine n-methyltransferase locus: a new model for studying the developmental distribution of adrenergic cells
Ebert, Steven N, et al.
Dev Dyn, 231:849-858 (2004)  2004

15517585 15517585
Postnatal development of the hyperpolarization-activated excitatory current Ih in mouse hippocampal pyramidal neurons
Vasilyev, Dmitry V and Barish, Michael E
J. Neurosci., 22:8992-9004 (2002)  2002

ImmunofluorescenceMouse12388606 12388606