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MAB360-25UL Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 (mouse monoclonal)

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers Gene Symbol: GFAP, FLJ45472 UniProt Number: P14136
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MAB360-25UL
25 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, H, M, Po, R, RbICC, IHC, IH(P), WBMAscitesMonoclonal Antibody
Description
Catalogue NumberMAB360-25UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5 (mouse monoclonal)
Alternate Names
  • GFAP
Background InformationGlial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
References
Product Information
FormatAscites
Control
  • Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
PresentationAscites fluid. Liquid. Contains no preservative.
Quality LevelMQ100
Applications
ApplicationAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunocytochemistry:
A previous lot was used on cultured mammalian cells.

Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.

Immunoblotting: A 1:1000 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user
Biological Information
ImmunogenPurified GFAP from porcine spinal cord.
CloneGA5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityGlial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
IsotypeIgG1
Species Reactivity
  • Bovine
  • Chicken
  • Human
  • Mouse
  • Pig
  • Rat
  • Rabbit
Species Reactivity NoteReacts with Bovine, Chicken, Human, Mouse, Porcine (Pig), Rabbit and Rat. Reactivity with other species has not been determined.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Gene Symbol
  • GFAP
  • FLJ45472
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
Molecular Weight~ 51 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB360-25UL 04054839337673

Documentation

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 (mouse monoclonal) SDS

Title

Safety Data Sheet (SDS) 

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 (mouse monoclonal) Certificates of Analysis

TitleLot Number
Anti-Glial Fibrillary Acidic Protein, clone GA5 (mouse monoclonal) - 3601711 3601711
Anti-Glial Fibrillary Acidic Protein, clone GA5 (mouse monoclonal) - 3303086 3303086
Anti-Glial Fibrillary Acidic Protein, clone GA5 (mouse monoclonal) - 3917384 3917384
Anti-Glial Fibrillary Acidic Protein, clone GA5 (mouse monoclonal) - 4034293 4034293
Anti-Glial Fibrillary Acidic Protein, clone GA5 (mouse monoclonal) - 4104142 4104142
Anti-Glial Fibrillary Acidic Protein, clone GA5 (mouse monoclonal) - 4127455 4127455
Anti-Glial Fibrillary Acidic Protein, clone GA5 (mouse monoclonal) Monoclonal Antibody Q2909944A