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AB5699 Anti-Cysteine-rich Motor Neuron 1 Antibody

Anti-Cysteine-rich Motor Neuron 1 Antibody detects level of Cysteine-rich Motor Neuron 1 & has been published & validated for use in IP & WB.

Anti-Cysteine-rich Motor Neuron 1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurochemistry & Neurotrophins Gene Symbol: CRIM1, CRIM-1, MGC138194, S52 UniProt Number: Q9NZV1
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AB5699
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIP, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberAB5699
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Cysteine-rich Motor Neuron 1 Antibody
Alternate Names
  • CRIM1
References
Product Information
FormatSerum
PresentationRabbit serum. Liquid.
Quality LevelMQ100
Applications
ApplicationAnti-Cysteine-rich Motor Neuron 1 Antibody detects level of Cysteine-rich Motor Neuron 1 & has been published & validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern blot: 1:1,000 using ECL. The antibody reacts with proteins of ~120 kDa. Preferred gel percentage is 7.5%. Suggested blocking buffer is PBS containing 5% non-fat milk. Suggested dilution buffer is PBS containing 1% non-fat milk.

Immunoprecipitation: 5 μL to 1 mL of cell extract. Preferred tissue/cell lysis buffer is RIPA. Suggested incubation time is 2-4 hours. Suggested capture agent is Protein A.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenRecombinant protein from the cytoplasmic tail of mouse Crim1.
HostRabbit
SpecificityCrim1

SUBCELLULAR LOCALIZATION: Intracellular and Membrane associated.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryMotor neurons are among the earliest neurons to appear after the commencement of cell patterning and the beginning of cell differentiation. Differentiation occurs in a ventral-to-dorsal gradient and is mediated, at least in part, by the concentration of ventrally expressed sonic hedgehog protein (SHH; MIM 600725). Dorsally expressed factors, such as members of the bone morphogenic protein (e.g., BMP4; MIM 112262) and transforming growth factor-beta (e.g., TGFB1; MIM 190180) families, can repress the induction of these neurons. CRIM1 may interact with growth factors implicated in motor neuron differentiation and survival (Kolle et al., 2000 [PubMed 10642437]).[supplied by OMIM]
Gene Symbol
  • CRIM1
  • CRIM-1
  • MGC138194
  • S52
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9NZV1 # May play a role in CNS development by interacting with growth factors implicated in motor neuron differentiation and survival. May play a role in capillary formation and maintenance during angiogenesis. Modulates BMP activity by affecting its processing and delivery to the cell surface.
SIZE: 1036 amino acids; 113738 Da
SUBUNIT: Interacts with BMP4 and BMP7.
SUBCELLULAR LOCATION: Processed cysteine-rich motor neuron 1 protein: Secreted. & Cell membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in pancreas, kidney, skeletal muscle, lung, placenta, brain, heart, spleen, liver and small intestine. Expressed in blood vessels (at protein level).
PTM: N-glycosylated.
SIMILARITY: SwissProt: Q9NZV1 ## Contains 4 antistasin-like domains. & Contains 1 IGFBP N-terminal domain. & Contains 6 VWFC domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5699 04053252330490