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07-729-25UL Anti-CTCF (rabbit antiserum)

Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.

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07-729-25UL
25 µL  
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Description
Catalogue Number07-729-25UL
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-CTCF (rabbit antiserum)
Alternate Names
  • 11 zinc finger transcriptional repressor
  • 11-zinc finger protein
  • CCCTC-binding factor
  • CCCTC-binding factor (zinc finger protein)
  • CTCFL paralog
  • transcriptional repressor CTCF
Background InformationCTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.
References
Product Information
FormatSerum
Control
  • HeLa nuclear extract.

    Included Positive Antigen Control:
    Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
PresentationRabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.
Applications
ApplicationUse Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor.
Key Applications
  • Western Blotting
  • ChIP-seq
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation:
An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes CTCF at a.a. 659-675.
Species Reactivity
  • Human
  • Rat
  • Canine
  • Mouse
  • Primate
Species Reactivity NoteHuman. Predicted to cross-react with mouse, rat, dog and primates based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.
Gene Symbol
  • CTCF
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION:SwissProt: P49711 # Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.
SIZE: 727 amino acids; 82785 Da
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes.
SIMILARITY: SwissProt: P49711 ## Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers.
Molecular WeightApprox. 120 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot.

Western Blot Analysis:
A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-729-25UL 04054839337413

Documentation

Anti-CTCF (rabbit antiserum) SDS

Title

Safety Data Sheet (SDS) 

Anti-CTCF (rabbit antiserum) Certificates of Analysis

TitleLot Number
Anti-CTCF (rabbit antiserum) - 3207491 3207491
Anti-CTCF (rabbit antiserum) - 3215503 3215503
Anti-CTCF (rabbit antiserum) - 3225874 3225874
Anti-CTCF (rabbit antiserum) - 3275487 3275487
Anti-CTCF (rabbit antiserum) - 3388643 3388643
Anti-CTCF (rabbit antiserum) - 3861827 3861827
Anti-CTCF (rabbit antiserum) - 3927990 3927990
Anti-CTCF (rabbit antiserum) - 4123394 4123394
Anti-CTCF (rabbit antiserum) - 4163469 4163469
Anti-CTCF (rabbit antiserum) - 4187263 4187263

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