Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE1952 Anti-CRTC1

Anti-CRTC1 Antibody, Cat. No. ABE1952, is a highly specific rabbit polyclonal antibody that has been validated for use in Immunoprecipitation and Western Blotting.

View Products on Sigmaaldrich.com
ABE1952
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABE1952
DescriptionAnti-CRTC1
Alternate Names
  • CREB-regulated transcription coactivator 1
  • Mucoepidermoid carcinoma translocated protein 1
  • Transducer of regulated cAMP response element-binding protein 1
  • TORC-1
  • Transducer of CREB protein 1
Background InformationCREB-regulated transcription coactivator 1 (UniProt: Q6UUV9; also known as Mucoepidermoid carcinoma translocated protein 1;Transducer of regulated cAMP response element-binding protein 1;TORC-1;Transducer of CREB protein 1) is encoded by the CRTC1 (also known as MECT1; TORC1; WAMTP1; KIAA0616) gene (Gene ID 23373) in human. CRTC1 is a 67.3 kDa protein that activates transcription through binding cAMP response element (CRE) sites on DNA. It is highly expressed in adult and fetal brain and is localized to specific regions such as the prefrontal cortex and cerebellum. Very low expression is observed in heart, spleen, lung, skeletal muscle, salivary gland, ovary, and kidney. Phosphorylation/dephosphorylation states of Ser151 are required for regulating transduction of CREB activity. It is mainly localized in the cytoplasm when phosphorylated by salt-induced kinase (SIK) or AMPK and when sequestered by 14-3-3 proteins. It is translocated to the nucleus upon dephosphorylation on Ser151. CRTC1 is shown to mediate early beta-amyloid-induced transcriptional and memory deficits and overexpression of CRTC1 is shown to rescue amyloid-induced transcriptional and cognitive deficits. CRTC1 is reported to ameliorate hippocampal-dependent learning and memory deficits in APPSw,Ind mice by enhancing the expression of a specific subset of CCTC1 target genes. A chromosomal aberration involving CRTC1 has been reported in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CRTC1 Antibody, Cat. No. ABE1952, is a highly specific rabbit polyclonal antibody that has been validated for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A 1:2,000 dilution from a representative lot detected CRTC1 in 10 µg of rat hippocampus lysate.

Immunoprecipitation Analysis: 4 µL from a representative lot immunoprecipitated exogenously expressed mouse CRTC1 from transfected HEK293 cells (Courtesy of Dr Carlos A. Saura, Institut de Neurociències, Universitat Autònoma de Barcelona, Spain).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected exogenously expressed mouse and rat CRTC1 in transfected HEK293 cells, as well as endogenous CRTC1 in mouse and rat hippocampus tissue homogenates (Courtesy of Dr Carlos A. Saura, Institut de Neurociències, Universitat Autònoma de Barcelona, Spain).
Biological Information
ImmunogenGST-tagged recombinat 135-amino acid fragment from the N-terminal region of human CRTC1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityABE1952 detected a target band in lysates from mouse and rat CRTC1-transfected HEK293 cells.
Species Reactivity
  • Rat
  • Mouse
Species Reactivity NoteMouse, Rat. Predicted to react with Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CRTC1
  • MECT1
  • TORC1
  • WAMTP1
  • KIAA0616
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~78 kDa observed. 67.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse hippocampus tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CRTC1 in 10 µg of mouse hippocampus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1952 04054839135255

Related Products & Applications