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ABT119 Anti-CELSR1 Antibody

This Anti-CELSR1 Antibody is validated for use in Western Blotting, IHC, Immunofluorescence for the detection of CELSR1.

Anti-CELSR1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: CELSR1, CDHF9, FMI2 UniProt Number: Q9NYQ6
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ABT119
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT119
DescriptionAnti-CELSR1 Antibody
Alternate Names
  • Cadherin EGF LAG seven-pass G-type receptor 1
  • Cadherin family member 9
  • Flamingo homolog 2
  • hFmi2
Background InformationThe Flamingo/CELSR family of 7TM-cadherins are characterized with a symmetric protein distribution and polarized activity at neighboring epithelial cell interfaces along defined axes of planar cell polarity. As a component of the extracellular matrix, the CELSR1 protein has been found particularly at the basal surface of neuroepithelial cells within both the early neural tube and a less well-defined group of ventricular zone cells at the midline of the developing spinal cord. CELSR1 plays a role in orienting hair follicles along the anterior–posterior axis of the developing mouse epidermis, both during embryogenesis and postnatal development.
References
Product Information
FormatAffinity Purified
Control
  • PC12 cell lysate.
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-CELSR1 Antibody is validated for use in Western Blotting, IHC, Immunofluorescence for the detection of CELSR1.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesImmunofluorescence Analysis: A 1:500 and 1:1,000 dilution from a representative lot detected CELSR1 in rat cerebellum, substancia nigra, and hippocampus tissue.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected CELSR1 in neurons of rat cortex tissue and in Purkinje cells and cells within the granular layer of rat cerebellum tissue.
Biological Information
ImmunogenLinear peptide corresponding to human CELSR1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Rat
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with Rat. Predicted to react with Human and Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic-type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like repeats and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact-mediated communication, with cadherin domains acting as homophilic binding regions and the EGF-like domains involved in cell adhesion and receptor-ligand interactions. This particular member is a developmentally regulated, neural-specific gene which plays an unspecified role in early embryogenesis.
Gene Symbol
  • CELSR1
  • CDHF9
  • FMI2
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Receptor that may have an important role in cell/cell signaling during nervous system formation.

SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.

POST-TRANSLATIONAL MODIFICATION: The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 9 cadherin domains. Contains 8 EGF-like domains. Contains 1 GPS domain. Contains 1 laminin EGF-like domain. Contains 2 laminin G-like domains.
Molecular Weight~330 kDa observed. A second isoform at ~152 kDa may be observed in some lysates.
An uncharacterized band at ~54 kDa may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected CELSR1 in 10 µg of PC12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT119 04053252643286