Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABC1123-100UG Anti-CEACAM5 (CD66e) Antibody, clone M5A

Anti-CEACAM5 (CD66e), clone M5A, Cat. No. MABC1123, is a humanized monoclonal antibody that detects Carcinoembryonic antigen-related cell adhesion molecule 5 and has been tested for use in Flow Cytometry, Immunohistochemistry, and Radioimmunoassay.

Anti-CEACAM5 (CD66e) Antibody, clone M5A MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: CEACAM5, CEA UniProt Number: P06731
View Products on Sigmaaldrich.com
MABC1123-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IHC, RIAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1123-100UG
DescriptionAnti-CEACAM5 (CD66e) Antibody, clone M5A
Alternate Names
  • Carcinoembryonic antigen-related cell adhesion molecule 5
  • Carcinoembryonic antigen
  • Meconium antigen 100
  • CD66e
Background InformationCarcinoembryonic antigen-related cell adhesion molecule 5 (UniProt: P06731; also known as Carcinoembryonic antigen, CEA, Meconium antigen 100, CD66e) is encoded by the CEACAM5 (also known as CEA) gene (Gene ID: 1048) in human. CEA is a cell surface heavily glycosylated homodimeric glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEA molecules can either be transmembrane or GPI-linked and are differentially expressed between species. CEA is restricted to the apical face of intestinal epithelial cells in the adult, but it has more diffuse distribution during embryonic development and in tumors. It functions as a calcium independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1. CEA participates in diverse physiological processes, including cell adhesion, differentiation, proliferation, and survival, as well as in carcinogenesis and bacterial pathogenesis. CEA molecules from neighboring cells can interact via their respective extracellular N-terminal IgV-like domain and mediate cell-cell adhesion through trans-oligomerization. CEA is synthesized with a signal peptide (aa 1-34) and a propeptide region (aa 686-702) that are cleaved to produce the mature form. It has seven Ig-like domains. Two isoforms of CEA have been reported that are produced by alternative splicing. Clone M5A is a humanized monoclonal antibody that displays high specificity and sub-nanomolar affinity for tumor-associated CEA. (Ref. Yazaki, PJ et al., (2004). Protein Eng. Des Sel. 17(5);481-489.
References
Product Information
FormatPurified
PresentationPurified humanized monoclonal antibody in PBS with 0.05% sodium azide. This humanized antibody was produced by transfecting the cDNA into murine myeloma NSO host cells for stable expression.
Applications
ApplicationAnti-CEACAM5 (CD66e), clone M5A, Cat. No. MABC1123, is a humanized monoclonal antibody that detects Carcinoembryonic antigen-related cell adhesion molecule 5 and has been tested for use in Flow Cytometry, Immunohistochemistry, and Radioimmunoassay.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Radioimmunoassay
Application NotesImmunohistochemistry Analysis: A representative lot detected CEACAM5 (CD66e) in Immunohistochemistry applications (Nittka, S., et. al. (2014). PLoS One. 9(9):e106921).

Radioimmunoassay Analysis: A representative lot detected CEACAM5 (CD66e) in Radioimmunoassay applications (Nittka, S., et. al. (2014). PLoS One. 9(9):e106921; Yazaki, P.J., et. al. (2004). Protein Eng Des Sel. 17(5):481-9; Yazaki, P.J., et. al. (2013). Protein Eng Des Sel. 26(3):187-93).
Biological Information
ImmunogenFull length purified human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA).
CloneM5A
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone M5A is a humanized monoclonal antibody that detects human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA). Note: please use anti-human secondary antibody for detedtion.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CEACAM5
  • CEA
Purification MethodProtein A purified
UniProt Number
Molecular Weight76.80 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in HT29 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CEACAM5 (CD66e) in one million HT29 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1123-100UG 04054839482205