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217595 Anti-CD40L Mouse mAb (24-31)

Anti-CD40L, mouse monoclonal, clone 24-32, recognizes the ~36 kDa CD40L (gp39) on activated CD3+ human PBL. It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.

Anti-CD40L Mouse mAb (24-31) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-gp39

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217595
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
217595-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~36 kDa CD40L (gp39) protein on activated CD3+ human PBL.
Note: 1 T = 1 test.
Catalogue Number217595
Brand Family Calbiochem®
SynonymsAnti-gp39
References
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preservative0.5 mg/ml gentamycin sulfate
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Oledzka, G., et al. 2007. Mol. Immunol. 44, 741.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Neutralization Studies
Application NotesFlow Cytometry (10 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Immunoblotting (see application references)
Application CommentsThis antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x105 ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman sgp39 fusion protein
ImmunogenHuman
Clone24-31
HostMouse
Purity≥95% by SDS-PAGE
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
217595-100UG 04055977202885

Documentation

Anti-CD40L Mouse mAb (24-31) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD40L Mouse mAb (24-31) Certificates of Analysis

TitleLot Number
217595
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-gp39
ApplicationFlow Cytometry (10 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Immunoblotting (see application references)
DescriptionProtein A affinity purified mouse monoclonal antibody. Recognizes the ~39-40 kDa CD40L protein.
BackgroundHuman CD40 ligand (CD40L) is a member of the tumor necrosis factor (TNF) family expressed on the surface of activated T-cells.
HostMouse
Immunogen speciesHuman
Immunogenhuman sgp39 fusion protein
Clone24-31
IsotypeIgG₁
Specieshuman
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Preservative0.5 mg/ml gentamycin sulfate
CommentsThis antibody immunoprecipitates the ~39 kDa CD40L (gp39). It will stain activated CD3+ human PBL and will functionally block MLR, spg39 induced B-cell proliferation and T-cell dependent B-cell differentiation. FACS was performed on 5x105 ficoll-prepared PHA/PMA stimulated human peripheral white blood cells using anti-CD40L at 10 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
Application referencesImmunoblotting Oledzka, G., et al. 2007. Mol. Immunol. 44, 741.