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MABF579 Anti-CD4 Antibody (human), APC-Cy7, clone RPA-T4

This mouse monoclonal antibody Anti-CD4 (human), APC-Cy7, clone RPA-T4 validated for use in IP, IHC & Flow Cytometry for the detection of CD4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD4 UniProt Number: P01730
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MABF579
25 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIP, IHC, FCMAPC-Cy7Monoclonal Antibody
Description
Catalogue NumberMABF579
DescriptionAnti-CD4 Antibody (human), APC-Cy7, clone RPA-T4
Alternate Names
  • T-cell surface glycoprotein CD4
  • T-cell surface antigen T4/Leu-3
  • CD antigen CD4
Background InformationThe RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).
References
Product Information
FormatAPC-Cy7
PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal antibody Anti-CD4 (human), APC-Cy7, clone RPA-T4 validated for use in IP, IHC & Flow Cytometry for the detection of CD4.
Key Applications
  • Immunoprecipitation
  • Immunohistochemistry
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneRPA-T4
Concentration5 μL (0.5 μg)/test
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CD4
Purification MethodSize Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg of this antibody detected CD4 in one million human peripheral blood lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF579 04053252963704

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies