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MABF546 Anti-CD25 Antibody (human), PE, clone BC96

This mouse monoclonal antibody Anti-CD25 (human), PE & clone BC96 validated for use in Flow Cytometry for the detection of CD25.

Anti-CD25 Antibody (human), PE, clone BC96 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: IL2RA UniProt Number: P01589
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MABF546
25 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPEMonoclonal Antibody
Description
Catalogue NumberMABF546
DescriptionAnti-CD25 Antibody (human), PE, clone BC96
Alternate Names
  • Interleukin-2 receptor subunit alpha
  • IL-2 receptor subunit alpha
  • IL-2-RA
  • IL-2R subunit alpha
  • IL2-RA
  • TAC antigen
  • p55
  • CD antigen CD25
Background InformationThe BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.
The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.
References
Product Information
FormatPE
PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal antibody Anti-CD25 (human), PE & clone BC96 validated for use in Flow Cytometry for the detection of CD25.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneBC96
Concentration5 µL (0.06 µg)/test
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • IL2RA
Purification MethodSize Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected CD25 in one million human peripheral blood lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF546 04053252963124