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MABF3124-25UL Anti-CCR8 Antibody, clone C8Mab-2

Anti-CCR8, clone C8Mab-2, Cat. No. MABF3124, is a rat monoclonal antibody that detects CCR8 and is tested for use in Flow Cytometry and Immunocytochemistry.

MABF3124-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3124-25UL
DescriptionAnti-CCR8 Antibody, clone C8Mab-2
Alternate Names
  • C-C chemokine receptor type 8
  • C-C CKR-8
  • CC-CKR-8
  • CCR-8
  • CDw198
Background InformationC-C chemokine receptor type 8 (UniProt: P56484; also known as C-C CKR-8; CC-CKR-8; CCR-8, CDw198) is encoded by the Ccr8 (also known as Cmkbr8, Ter1) gene (Gene ID: 12776) in murine species. CCR8, a member of the G-protein coupled receptor 1 family, is a multi-pass membrane protein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. Its expression is observed in thymus and its higher expression is observed in Treg and T helper 2 (TH2) cells. It is recruited to the inflammation site and has been implicated in allergy and asthma. It serves as a receptor for CCL1/SCY1 chemokine. In human and in murine models CCR8+ Tregs account for 30 to 80% of tumor Tregs in various cancers and only less than 10% in other tissues. However, most tumor-infiltrating conventional T cells are reported to be CCR8 negative. Disruption of CCR8 function is reported to result in reduced accumulation of Treg cells within tumors and disrupts their immunosuppressive function. (Ref.: Kidani, Y., et al. (2022). Proc. Natl. Acad. Sci. USA. 119(7); e2114282119; Tanaka, T., et al. (2021). Monoclonal. Antib. Immunodiagn. Immunother. 40(2); 65-70; Zingoni, A., et al. (1998). J. Immunol. 161(2); 547-551).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-CCR8, clone C8Mab-2, Cat. No. MABF3124, is a rat monoclonal antibody that detects CCR8 and is tested for use in Flow Cytometry and Immunocytochemistry.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Application NotesTested applications

Immunocytochemistry Analysis: A representative lot detected CCR8 in Immunocytochemistry applications (Saito, M., et al. (2022). Monoclon Antib Immunodiagn Immunother. 41(2):115-119).

Flow Cytometry Analysis: A representative lot detected CCR8 in Flow Cytometry applications (Tanaka, T., et al. (2021). Monoclon Antib Immunodiagn Immunother. 40(2):65-70; Saito, M., et al. (2022). Monoclon Antib Immunodiagn Immunother. 41(2):115-119).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenChinese Hamster Ovary (CHO) cells stably expressing mouse CCR8.
EpitopeExtracellular domain
CloneC8Mab-2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone C8Mab-2 is a rat monoclonal antibody that detects C-C chemokine receptor type 8 (CCR8). It targets an epitope within the extracellular domain.
IsotypeIgG2b
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ccr8
Purification MethodProtein G purified
UniProt Number
Molecular Weight40.05 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Chinese Hamster ovary (CHO) cells stably expressing murine CCR8.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CCR8 in one million Chinese Hamster ovary CHO) cells stably expressing murine CCR8.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3124-25UL 04065270172525