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AB1735 Anti-Bid Antibody

Detect Bid using this Anti-Bid Antibody validated for use in WB, IP, IH.

Anti-Bid Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: BID, FP497, MGC42355, MGC15319 UniProt Number: P55957
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AB1735
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, RbWB, IP, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberAB1735
Replaces04-438
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Bid Antibody
Background InformationBid, a BH3 domain-containing proapoptotic Bcl-2 family member, is known to interact with Bcl-2 and Bax. It has been reported that Bid induces cytochrome c release from mitochondria following cleavage by caspase-8 in response to apoptotic stimuli transmitted through cell surface death receptors. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
References
Product Information
FormatSerum
PresentationWhole serum without preservatives
Quality LevelMQ100
Applications
ApplicationDetect Bid using this Anti-Bid Antibody validated for use in WB, IP, IH.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
Application NotesWestern blot: Recommended antibody dilution 1:3,000 to 1:6,000. Recognizes a band migrating at approximately 22 kDa corresponding to Bid.

Immunoprecipitation: Recommended antibody dilution 1:200

Immunohistochemistry: Recommended antibody dilution 1:1500

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenRecombinant full length GST-tagged mouse Bid protein
HostRabbit
SpecificityAB1735 recognizes Bid from human, mouse, rat and rabbit.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Rabbit
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Gene Symbol
  • BID
  • FP497
  • MGC42355
  • MGC15319
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P55957 # The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
SIZE: 195 amino acids; 21995 Da
SUBUNIT: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion membrane (By similarity). Note=When uncleaved, it is predominantly cytoplasmic. & BH3-interacting domain death agonist p15: Mitochondrion membrane (By similarity). Note=Translocates to mitochondria as an integral membrane protein (By similarity). & BH3-interacting domain death agonist p13: Mitochondrion membrane (By similarity). Note=Associated with the mitochondrial membrane (By similarity). & Isoform 1: Cytoplasm. & Isoform 3: Cytoplasm. & Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
TISSUE SPECIFICITY: Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
DOMAIN: SwissProt: P55957 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products (By similarity). & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY:
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 12 months.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB1735 04053252742422