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MABC1597-25UG Anti-Bax Antibody, clone 3C10

Detect using this rat monoclonal Anti-Bax, clone 3C10, Cat. No. MABC1597, is a rat monoclonal antibody that detects Apoptosis regulator BAX and has been tested for use in Immunoprecipitation, Inhibition/Function Analysis, and Western Blotting.

MABC1597-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1597-25UG
DescriptionAnti-Bax Antibody, clone 3C10
Alternate Names
  • Apoptosis regulator BAX
  • Bcl-2-like protein 4
  • Bcl2-L-4
Background InformationApoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. Bax displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Bax is largely cytosolic under normal conditions, but upon induction of apoptosis it undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. Bax is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 3C10 that is specific to the Bax 1- 2 loop activates mitochondrial Bax, but blocks translocation of cytosolic Bax. It recognizes a peptide sequence at the start of Bax 1 2 loop and can bind mitochondrial Bax to induce dimerization and cytochrome c release. (Ref.: Iyer, S., et al. (2016). Nature Commun. 7; 11734).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
Applications
ApplicationDetect using this rat monoclonal Anti-Bax, clone 3C10, Cat. No. MABC1597, is a rat monoclonal antibody that detects Apoptosis regulator BAX and has been tested for use in Immunoprecipitation, Inhibition/Function Analysis, and Western Blotting.
Key Applications
  • Immunoprecipitation
  • Inhibits Activity/Function
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Bax in Immunoprecipitation applications (Iyer, S., et. al. (2016). Nat Commun. 7:11734).

Inhibits Activity/Function Analysis: A representative lot prevented tBid-triggered Bax translocation and cytochrome c release. (Iyer, S., et. al. (2016). Nat Commun. 7:11734).

Western Blotting Analysis: A representative lot detected Bax in Western Blotting applications (Iyer, S., et. al. (2016). Nat Commun. 7:11734).

Induces Function Analysis: A representative lot of this antibody activated mitochondrial Bax. (Iyer, S., et. al. (2016). Nat Commun. 7:11734).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region.
Clone3C10
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 3C10 is a rat monoclonal antibody that detects human and mouse Bax, It targets an epitope within the alpha 1 and 2 loop in the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BAX
  • BCL2L4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~23 kDa observed; 21.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in wild type (WT) HeLa cells and Bax/Bak double knockout HeLa cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Bax in lysate from wild type (WT) HeLa cells, but not in lysate from Bax/Bak double knockout HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1597-25UG 04054839646720