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MABC46 Anti-Atg13 Antibody, clone 2H4.2

This Anti-Atg13 Antibody, clone 2H4.2 is validated for use in Western Blotting, IHC for the detection of Atg13.

Anti-Atg13 Antibody, clone 2H4.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: ATG13 UniProt Number: O75143
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MABC46
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC46
DescriptionAnti-Atg13 Antibody, clone 2H4.2
Alternate Names
  • Autophagy-related protein 13
Background InformationThe autophagy-related protein 13 (Atg13) plays an important role in the formation of autophagosomes. Autophagosomes are formed in response to nutrient deprivation and function as the transport vesicles for organelles, proteins, and protein complexes targeted for lysosomes that digest these cargos to produce energy and nutrients. Atg13 is activated by the mTOR pathway and forms a complex with the FIP200 protein. This complex is involved in enhancing the activity of the ULK1 kinase which is required for the formation of autophagosomes. Atg13/FIP200 faciliates the localization of ULK1 to pre-autophagosomes, and subsequently stabilizes ULK1. Autophagy is an important process in development, growth, and cell differentiation, and disruption of this process may contribute to cancer, aging, and neurodegenerative diseases.
References
Product Information
FormatPurified
Control
  • Campthothecin treated HeLa cell lysate.
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Atg13 Antibody, clone 2H4.2 is validated for use in Western Blotting, IHC for the detection of Atg13.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blot Analysis: A representative lot detected Atg13 in HeLa cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Atg13.
Clone2H4.2
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATG13
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~60 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in campthothecin treated HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 µg of campthothecin treated HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC46 04053252522079

Documentation

Anti-Atg13 Antibody, clone 2H4.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-Atg13 Antibody, clone 2H4.2 Certificates of Analysis

TitleLot Number
Anti-Atg13, clone 2H4.2 - 2367819 2367819
Anti-Atg13, clone 2H4.2 - 2379491 2379491
Anti-Atg13, clone 2H4.2 - 2055306 2055306
Anti-Atg13, clone 2H4.2 - 2296762 2296762
Anti-Atg13, clone 2H4.2 - 2328388 2328388
Anti-Atg13, clone 2H4.2 - 2360590 2360590
Anti-Atg13, clone 2H4.2 - 3429920 3429920
Anti-Atg13, clone 2H4.2 - 3488524 3488524
Anti-Atg13, clone 2H4.2 - Q2009200 Q2009200
Anti-Atg13, clone 2H4.2 -2489998 2489998
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Brochure

Title
New Products: Volume 3, 2012

Newsletters / Publications

Title
Autophagy Research Focus