Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS45 Anti-Annexin A1 Antibody, clone CPTC-ANXA1-2

This Anti-Annexin A1 Antibody, clone CPTC-ANXA1-2 is validated for use in WB, IH for the detection of Annexin A1.

Anti-Annexin A1 Antibody, clone CPTC-ANXA1-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: GPCR, cAMP/cGMP & Calcium Signaling Gene Symbol: ANXA1, LPC1, ANX1, p35 UniProt Number: P04083
View Products on Sigmaaldrich.com
MABS45
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS45
DescriptionAnti-Annexin A1 Antibody, clone CPTC-ANXA1-2
Alternate Names
  • annexin A1
  • chromobindin-9
  • annexin I (lipocortin I)
  • Lipocortin I
  • calpactin-2
  • Calpactin II
  • annexin-1
  • Phospholipase A2 inhibitory protein
  • Annexin I
  • Calpactin-2
  • Annexin-13
  • Chromobindin-9
Background InformationAnnexin A1 (ANXA1) is a calcium and phospholipid binding protein almost entirely restricted to microglia in the CNS. During the peripheral inflammatory response, Annexin A1 is instrumental in the phagocytosis of apoptotic leukocytes. Annexin A1 restores the ability of microglia to differentiate between apoptotic and non-apoptotic cells during inflammatory activation. Current research is geared towards using Annexin A1 as a therapeutic target in neuroinflammation. Annexin A1 has also been shown to be influential in cancer development and proliferation.
References
Product Information
FormatPurified
Control
  • K562 cell lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Annexin A1 Antibody, clone CPTC-ANXA1-2 is validated for use in WB, IH for the detection of Annexin A1.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (http://antibodies.cancer.gov/antibodies/detail/CPTC-ANXA1-2)
Biological Information
ImmunogenFull length recombinant protein corresponding to human Annexin A1.

Material obtained from the National Cancer Institute’s Clinical Proteomic Technologies for Cancer (CPTC) program
CloneCPTC-ANXA1-2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAnnexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq].
Gene Symbol
  • ANXA1
  • LPC1
  • ANX1
  • p35
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

SUBUNIT STRUCTURE: Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF (By similarity).

SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Cell projection › cilium (By similarity). Basolateral cell membrane (By similarity). Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages (By similarity).

DOMAIN: A pair of annexin repeats may form one binding site for calcium and phospholipid.

PTM: Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.

SEQUENCE SIMILARITIES: Belongs to the annexin family.

Contains 4 annexin repeats.
Molecular Weight~ 39 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Annexin A1 on 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS45 04053252644870

Documentation

Anti-Annexin A1 Antibody, clone CPTC-ANXA1-2 SDS

Title

Safety Data Sheet (SDS) 

Anti-Annexin A1 Antibody, clone CPTC-ANXA1-2 Certificates of Analysis

TitleLot Number
Anti-Annexin A1, clone CPTC-ANXA1-2 - 2450742 2450742
Anti-Annexin A1, clone -2650874 2650874
Anti-Annexin A1, clone -2673126 2673126
Anti-Annexin A1, clone CPTC-ANXA1-2 - 2051422 2051422
Anti-Annexin A1, clone CPTC-ANXA1-2 - 3660416 3660416
Anti-Annexin A1, clone CPTC-ANXA1-2 - 4048711 4048711
Anti-Annexin A1, clone CPTC-ANXA1-2 - NRG1831974 NRG1831974
Anti-Annexin A1, clone CPTC-ANXA1-2 Monoclonal Antibody 3128123