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ABS1023 Anti-Angiomotin Antibody

Anti-Angiomotin Antibody | ABS1023 is an antibody against Angiomotin Antibody for use in Western Blotting, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP).

Anti-Angiomotin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: AMOT UniProt Number: Q4VCS5
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ABS1023
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, IF, ICC, ChIPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1023
DescriptionAnti-Angiomotin Antibody
Alternate Names
  • Angiomotin
Background InformationAngiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Angiomotin Antibody | ABS1023 is an antibody against Angiomotin Antibody for use in Western Blotting, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP).
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
Application NotesWestern Blotting Analysis: A representative lot detected phospho Angiomotin (Ser175) in HEK293 transfected with AMOT cell lysate (Dr. Joseph Kissil, Scripps Research Institute, Florida).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells expressing equal amount of HA-Merlin and Myc-Rich1, untreated (−) or Doxycyline induced (+) RT4-67 cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
Western Blotting Analysis: A representative lot detected Angiomotin in Flag-IP and total cell lysate from HEK293 cells transfected with Flag-tagged wild-type (WT) or patient-derived mutant Merlin alleles, HEK293, ERK activation in Nf2−/− SC4 cells or Merlin-reconstituted SC4 cells (Nf2−/−+retroNf2) (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
Western Blotting Analysis: A representative lot detected Angiomotin in HEK293, MEFs treated with Adeno (−) or
Adeno-Cre (+) virus (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
Immunofluorescence Analysis: A representative lot detected Angiomotin in epithelial cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).
Immunofluorescence Analysis: A representative lot detected Angiomotin in liver from Amot KO mice (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
Immunocytochemistry Analysis: A representative lot detected Angiomotin in SC4 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
Biological Information
ImmunogenLinear peptide corresponding to human Angiomotin.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody detects p80 and p130 forms of AMOT
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • AMOT
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight150/80 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 transfected with LATS cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Angiomotin in 10 µg of HEK293 transfected with LATS cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1023 04055977126938