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ABT1485 Anti-Actin

This rabbit polyclonal Anti-Actin Antibody, Cat. No. ABT1485 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Actin.

Anti-Actin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ACTA1, ACTA, CFTD, NEM3, MPFD UniProt Number: P68133
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ABT1485
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, IH(P), WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT1485
DescriptionAnti-Actin
Alternate Names
  • Actin
  • alpha skeletal muscle
  • Alpha-actin-1
Background InformationActins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show >90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic - and -actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rabbit polyclonal Anti-Actin Antibody, Cat. No. ABT1485 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Actin.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in A431 and NIH/3T3 cells.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected actin in human cardiac muscle, skeletal muscle, and small intestine tissue sections.

This polyclonal antibody is also available as Alexa Fluor® 488 and Alexa Fluor® 647 conjugates (Cat. No. ABT1485-AF488 and ABT1485-AF647) for flow cytometry, immunocytochemistry, immunofluorescence applications.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody was raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ACTA1
  • ACTA
  • CFTD
  • NEM3
  • MPFD
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~42.0 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected actin in 10 µg of human skeletal muscle tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT1485 04054839055515