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ABE1320 Anti-ASXL2

Anti-ASXL2, Cat. No. ABE1320, is a highly specific rabbit polyclonal antibody that targets Additional sex combs-like protein 2 and has been tested in Immunocytochemistry and Western Blotting.

Anti-ASXL2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ASXL2, ASXH2, KIAA1685 UniProt Number: Q76L83
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ABE1320
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE1320
DescriptionAnti-ASXL2
Alternate Names
  • Putative Polycomb group protein ASXL2
  • Additional sex combs-like protein 2
Background InformationPutative Polycomb group protein ASXL2 (UniProt: Q76L83; also known as Additional sex combs-like protein 2) is encoded by the ASXL2 (also known as ASXH2, KIAA1685) gene (Gene ID: 55252) in human. ASXL2 is a putative Polycomb group (PcG) protein that acts by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. It is not required to initiate repression, but is essential to maintain it during later stages of development. They probably act via methylation of histones, rendering chromatin heritably changed in its expressibility. ASXL2 is shown to be involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as peroxisome proliferator-activated receptor gamma (PPARG). It also acts as coactivator for PPARG and enhances its adipocyte differentiation-inducing activity; the function seems to involve differential recruitment of acetylated and methylated histone H3. ASXL2 is reported to be essential for haematopoiesis and acts as a haploinsufficient tumor suppressor in leukemia. ASXL2 gene is one of most commonly mutated genes in AML patients bearing the AML1-ETO fusion oncoprotein and loss of ASXL2 is shown to promote AML1-ETO-mediated leukemogenesis. ASXL2 protein contains two LXXLL motifs (aa 303-307 and 887-891), which are required for an association with nuclear receptors. It also contains an atypical PHD-type zinc finger regions (aa 1397-1434). Two isoforms of ASXL2 are reported that are produced by alternative splicing. Mutation in ASXL2 gene have also been linked to the development of Shashi-Pena syndrome that is characterized by delayed psychomotor development, intellectual disability, and macrocephaly, and neonatal feeding difficulties. (Ref.: Micol, JB et al. (2017). Nat. Commun. DOI: 10.1038/ncomms15429).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ASXL2, Cat. No. ABE1320, is a highly specific rabbit polyclonal antibody that targets Additional sex combs-like protein 2 and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ASXL2 in HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 22 amino acids from the internal region of human Additional sex combs-like protein 2 (ASXL2).
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Additional sex combs-like protein 2 (ASXL2) in human cells. It targets an epitope within 22 amino acids from the internal region.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ASXL2
  • ASXH2
  • KIAA1685
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~154 kDa observed; 153.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected ASXL2 in 10 µg of Raji nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1320 04054839412349